DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Han, Yong-Mahn | - |
dc.contributor.advisor | 한용만 | - |
dc.contributor.author | Cho, Yun-Hee | - |
dc.contributor.author | 조윤희 | - |
dc.date.accessioned | 2015-04-23T02:08:41Z | - |
dc.date.available | 2015-04-23T02:08:41Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=568514&flag=dissertation | - |
dc.identifier.uri | http://hdl.handle.net/10203/196218 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2014.2, [ 109 p. ] | - |
dc.description.abstract | The pluripotency of embryonic stem cells (ESCs) is maintained by intracellular networks of many pluripotency-associated (PA) proteins such as OCT4, SOX2, and NANOG. However, the mechanisms underlying the regulation of protein homeostasis for pluripotency remain elusive. Here, we first demonstrate that autophagy acts together with the ubiquitin-proteasome system (UPS) to modulate the levels of PA proteins in human ESCs (hESCs). Autophagy inhibition impaired the pluripotency despite increment of PA proteins in hESCs. Immunogold-electron microscopy confirmed localization of OCT4 molecules within autophagosomes. Also, knockdown of LC3 expression led to accumulation of PA proteins and reduction of pluripotency in hESCs. Interestingly, autophagy and the UPS showed differential kinetics in the degradation of PA proteins. Autophagy inhibition caused enhanced accumulation of both cytoplasmic and nuclear PA proteins whereas the UPS inhibition led to preferentially degrade nuclear PA proteins. Our findings suggest that autophagy modulates homeostasis of PA proteins, providing a new insight in the regulation of pluripotency in hESCs. | eng |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | 유비퀴틴-프로테아좀 기작 | - |
dc.subject | 오토파지 | - |
dc.subject | 인간배아줄기세포 | - |
dc.subject | 항상성 유지 | - |
dc.subject | 전능성 | - |
dc.subject | Cooperative action | - |
dc.subject | Pluripotency | - |
dc.subject | Homeostasis | - |
dc.subject | Ub-proteasome system | - |
dc.subject | Autophagy | - |
dc.subject | Human embryonic stem cell | - |
dc.subject | pluripotency-associated proteins | - |
dc.title | The role of autophagy in the self-renewal of human pluripotent stem cells | - |
dc.title.alternative | 인간전분화성 줄기세포에서 자가재생산에 기여하는 오토파지의 역할 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 568514/325007 | - |
dc.description.department | 한국과학기술원 : 생명과학과, | - |
dc.identifier.uid | 020078035 | - |
dc.contributor.localauthor | Han, Yong-Mahn | - |
dc.contributor.localauthor | 한용만 | - |
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