재조합 대장균에서 MaoC를 이용한 지방산으로부터의 중간사슬길이 폴리하이드록시알칸산 생산 연구MaoC Mediated Biosynthesis of Medium-chain-length Polyhydroxyalkanoates in Recombinant Escherichia coli from Fatty Acid

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Biosynthesis pathway of medium-chain-length(MCL) polyhydroxyalkanoates (PHA) from fatty acid β-oxidationpathway was constructed in recombinant Escherichiacoli by introducing the Pseudomonas sp. 61-3 PHA synthasegene (phaC2) and the maoC genes from Pseudomonas putida,Sinorhizobium meliloti, and Ralstonia eutropha. The metaboliclink between fatty acid β-oxidation pathway and PHAbiosynthesis pathway was constructed by MaoC, which ishomologous to P. aeruginosa (R)-specific enoyl-CoA hydratase(PhaJ1). When the E. coli W3110 strains expressing thephaC2 gene and one of the maoC genes from P. putida, Sinorhizobiummeliloti, and Ralstonia eutropha were cultured inLB medium containing 2 g/L of sodium decanoate as a carbonsource, MCL-PHA that mainly consists of 3-hydroxyhexanoate(3HHx), 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate(3HD), was produced. The monomer composition ofPHA and PHA contents varied depending on MaoC employedfor the production of PHA. The highest PHA content of 18.7wt% was achieved in recombinant E. coli W3110 expressingthe phaC2 gene and the P. putida maoC gene. These resultssuggest that MCL-PHA biosynthesis pathway can be constructedin recombinant E. coli strains from the b-oxidation pathwayby employing MaoC able to supply (R)-3-hydroxyacyl-CoA, the substrate of PHA synthase.
Publisher
한국생물공학회
Issue Date
2014-08
Language
Korean
Citation

KSBB JOURNAL, v.29, no.4, pp.244 - 249

ISSN
1225-7117
URI
http://hdl.handle.net/10203/195711
Appears in Collection
CBE-Journal Papers(저널논문)
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