DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Seung-Kyoon | ko |
dc.contributor.author | Lee, Hosuk | ko |
dc.contributor.author | Han, Kyu Min | ko |
dc.contributor.author | Kim, Sang Cheol | ko |
dc.contributor.author | Choi, Yoonjung | ko |
dc.contributor.author | Park, Sang-Wook | ko |
dc.contributor.author | Bak, Geunu | ko |
dc.contributor.author | Lee, Younghoon | ko |
dc.contributor.author | Choi, Jung Kyoon | ko |
dc.contributor.author | Kim, Tae-Kyung | ko |
dc.contributor.author | Han, Yong-Mahn | ko |
dc.contributor.author | Lee, Daeyoup | ko |
dc.date.accessioned | 2015-04-07T02:42:10Z | - |
dc.date.available | 2015-04-07T02:42:10Z | - |
dc.date.created | 2014-12-30 | - |
dc.date.created | 2014-12-30 | - |
dc.date.created | 2014-12-30 | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | CELL STEM CELL, v.15, no.6, pp.735 - 749 | - |
dc.identifier.issn | 1934-5909 | - |
dc.identifier.uri | http://hdl.handle.net/10203/195119 | - |
dc.description.abstract | LIN28-mediated processing of the microRNA (miRNA) let-7 has emerged as a multilevel program that controls self-renewal in embryonic stem cells. LIN28A is believed to act primarily in the cytoplasm together with TUT4/7 to prevent final maturation of let-7 by Dicer, whereas LIN28B has been suggested to preferentially act on nuclear processing of let-7. Here, we find that SET7/9 monomethylation in a putative nucleolar localization region of LIN28A increases its nuclear retention and protein stability. In the nucleoli of human embryonic stem cells, methylated LIN28A sequesters pri-let-7 and blocks its processing independently of TUT4/7. The nuclear form of LIN28A regulates transcriptional changes in MYC-pathway targets, thereby maintaining stemness programs and inhibiting expression of early lineage-specific markers. These findings provide insight into the molecular mechanism underlying the post-translational methylation of nuclear LIN28A and its ability to modulate pluripotency by repressing let-7 miRNA expression in human embryonic stem cells. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | SET7/9 Methylation of the Pluripotency Factor LIN28A Is a Nucleolar Localization Mechanism that Blocks let-7 Biogenesis in Human ESCs | - |
dc.type | Article | - |
dc.identifier.wosid | 000347174300012 | - |
dc.identifier.scopusid | 2-s2.0-84919426973 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 735 | - |
dc.citation.endingpage | 749 | - |
dc.citation.publicationname | CELL STEM CELL | - |
dc.identifier.doi | 10.1016/j.stem.2014.10.016 | - |
dc.contributor.localauthor | Lee, Younghoon | - |
dc.contributor.localauthor | Choi, Jung Kyoon | - |
dc.contributor.localauthor | Han, Yong-Mahn | - |
dc.contributor.localauthor | Lee, Daeyoup | - |
dc.contributor.nonIdAuthor | Kim, Sang Cheol | - |
dc.contributor.nonIdAuthor | Kim, Tae-Kyung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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