Enhancement of xylitol production in glycerol kinase disrupted Candida tropicalis by co-expression of three genes involved in glycerol metabolic pathway

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Glycerol can be used as a primary carbon source by yeasts, little is known regarding glycerol metabolism in Candida tropicalis. In this study, glycerol kinase gene (gk) was disrupted from xylitol dehydrogenase gene (XYL2) knockout C. tropicalis strain BSXDH-3. The resultant gk knockout C. tropicalis strain was incapable to grow on glycerol. The cells growth on glycerol was resumed by co-expressing Scheffersomyces stipitis gcy1, 2 and 3 genes, which respectively encode NADP(+)-dependent glycerol dehydrogenase 1, 2 and 3, under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "GK", than in BSXDH-3. In fermentation experiments using glycerol as co-substrate with xylose, strain GK produced xylitol 0.85 and 1.28 g l(-1) h(-1) at the time periods of 16 and 24 h, respectively, which is 30 and 18 % higher at same time intervals in BSXDH-3. This is the first report of gk gene disruption and co-expression of gcy1, 2 and 3 genes for NADPH regeneration and enhanced xylitol production in C. tropicalis.
Publisher
SPRINGER
Issue Date
2013-09
Language
English
Article Type
Article
Keywords

COLD-INDUCED INCREASE; NEUROSPORA-CRASSA; GLYCEROPHOSPHATE DEHYDROGENASE; SACCHAROMYCES-CEREVISIAE; XYLOSE; FERMENTATION; MUTANT

Citation

BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.36, no.9, pp.1279 - 1284

ISSN
1615-7591
DOI
10.1007/s00449-012-0872-4
URI
http://hdl.handle.net/10203/193864
Appears in Collection
BS-Journal Papers(저널논문)
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