Metabolic engineering of Pichia pastoris for production of hyaluronic acid with high molecular weight

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The high molecular weight (>1 MDa) of hyaluronic acid (HA) is important for its biological functions. The reported limiting factors for the production of HA with high molecular weight (MW) by microbial fermentation are the insufficient HA precursor pool and cell growth inhibition. To overcome these issues, the Xenopus laevis xhasA2 and xhasB genes encoding hyaluronan synthase 2 (xhasA2) and UDP-glucose dehydrogenase (xhasB), were expressed in Pichia pastoris widely used for production of heterologous proteins. In this study, expression vectors containing various combination cassettes of HA pathway genes including xhasA2 and xhasB from X. laevis as well as UDP-glucose pyrophosphorylase (hasC), UDP-N-acetylglucosamine pyrophosphorylase (hasD) and phosphoglucose isomerase (hasE) from P. pastoris were constructed and tested. First, HA pathway genes were overexpressed using pAO815 and pGAPZB vectors, resulting in the production of 1.2 MDa HA polymers. Second, in order to decrease hyaluronan synthase expression a strong AOX1 promoter in the xhasA2 gene was replaced by a weak AOX2 promoter which increased the mean MW of HA to 2.1 MDa. Finally, the MW of HA polymer was further increased to 2.5 MDa by low-temperature cultivation (26 degrees C) which reduced cell growth inhibition. The yield of HA production by the P. pastoris recombinant strains in 1 L of fermentation culture was 0.8-1.7 g/L.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2014-09
Language
English
Article Type
Article
Keywords

GROUP-A STREPTOCOCCI; MICROBIAL-PRODUCTION; LACTOCOCCUS-LACTIS; ZOOEPIDEMICUS; SYNTHASES; CULTURE; OPERON

Citation

JOURNAL OF BIOTECHNOLOGY, v.185, pp.28 - 36

ISSN
0168-1656
DOI
10.1016/j.jbiotec.2014.05.018
URI
http://hdl.handle.net/10203/193825
Appears in Collection
BS-Journal Papers(저널논문)
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