DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, E | ko |
dc.contributor.author | Kim, K | ko |
dc.contributor.author | Yang, H | ko |
dc.contributor.author | Kim, YT | ko |
dc.contributor.author | Kwak, Juhyoun | ko |
dc.date.accessioned | 2010-09-08T09:06:01Z | - |
dc.date.available | 2010-09-08T09:06:01Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-11 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.75, no.21, pp.5665 - 5672 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/19336 | - |
dc.description.abstract | We have developed a sandwich-type enzyme-linked DNA sensor as a new electrochemical method to detect DNA hybridization. A partially ferrocenyl-tethered poly(amidoamine) dendrimer (Fc-D) was used as an electrocatalyst to enhance the electronic signals of DNA detection as well as a building block to immobilize capture probes. Fc-D was immobilized on a carboxylic acid-terminated self-assembled monolayer (SAM) by covalent coupling of unreacted amine in Fc-D to the acid. Thiolated capture probe was attached to the remaining amine groups of Fc-D on the SAM via a bifunctional linker. The target DNA was hybridized with the capture probe, and an extension in the DNA of the target was then hybridized with a biotinylated detection probe. Avidin-conjugated alkaline phosphatase was bound to the detection probe and allowed to generate the electroactive label, p-aminophenol, from p-aminophenyl phosphate enzymatically. p-Aminophenol diffuses into the Fc-D layer and is then electrocatalytically oxidized by the electronic mediation of the immobilized Fc-D, which leads to a great enhancement in signal. Consequently, the amount of hybridized target can be estimated using the intensity of electrocatalytic current. This DNA sensor exhibits a detection limit of 20 fmol. Our method was also successfully applied to the sequence-selective discrimination between perfectly matched and single-base mismatched target oligonucleotides. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | QUARTZ-CRYSTAL-MICROBALANCE | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | SURFACE-PLASMON RESONANCE | - |
dc.subject | NUCLEIC-ACID | - |
dc.subject | AMPEROMETRIC DETECTION | - |
dc.subject | ALKALINE-PHOSPHATASE | - |
dc.subject | MODIFIED ELECTRODE | - |
dc.subject | IMPROVED SUBSTRATE | - |
dc.subject | CARBON ELECTRODES | - |
dc.subject | MIXED MONOLAYERS | - |
dc.title | Enzyme-amplified electrochemical detection of DNA using electrocatalysis of ferrocenyl-tethered dendrimer | - |
dc.type | Article | - |
dc.identifier.wosid | 000186339000009 | - |
dc.identifier.scopusid | 2-s2.0-0242291133 | - |
dc.type.rims | ART | - |
dc.citation.volume | 75 | - |
dc.citation.issue | 21 | - |
dc.citation.beginningpage | 5665 | - |
dc.citation.endingpage | 5672 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kwak, Juhyoun | - |
dc.contributor.nonIdAuthor | Kim, E | - |
dc.contributor.nonIdAuthor | Kim, K | - |
dc.contributor.nonIdAuthor | Yang, H | - |
dc.contributor.nonIdAuthor | Kim, YT | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | QUARTZ-CRYSTAL-MICROBALANCE | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | SURFACE-PLASMON RESONANCE | - |
dc.subject.keywordPlus | NUCLEIC-ACID | - |
dc.subject.keywordPlus | AMPEROMETRIC DETECTION | - |
dc.subject.keywordPlus | ALKALINE-PHOSPHATASE | - |
dc.subject.keywordPlus | MODIFIED ELECTRODE | - |
dc.subject.keywordPlus | IMPROVED SUBSTRATE | - |
dc.subject.keywordPlus | CARBON ELECTRODES | - |
dc.subject.keywordPlus | MIXED MONOLAYERS | - |
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