DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sun, Jiali | ko |
dc.contributor.author | Jeon, Jaehyun | ko |
dc.contributor.author | Shin, Minsang | ko |
dc.contributor.author | Shin, Ho Chul | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.contributor.author | Kim, Jeong-Sun | ko |
dc.date.accessioned | 2015-01-27T02:04:21Z | - |
dc.date.available | 2015-01-27T02:04:21Z | - |
dc.date.created | 2014-04-01 | - |
dc.date.created | 2014-04-01 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.citation | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.70, pp.535 - 543 | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.uri | http://hdl.handle.net/10203/193058 | - |
dc.description.abstract | A multi-subunit ribonucleoprotein complex termed the Cmr RNA-silencing complex recognizes and destroys viral RNA in the CRISPR-mediated immune defence mechanism in many prokaryotes using an as yet unclear mechanism. In Archaeoglobus fulgidus, this complex consists of six subunits, Cmr1-Cmr6. Here, the crystal structure of Cmr1 from A. fulgidus is reported, revealing that the protein is composed of two tightly associated ferredoxin-like domains. The domain located at the N-terminus is structurally most similar to the N-terminal ferredoxin-like domain of the CRISPR RNA-processing enzyme Cas6 from Pyrococcus furiosus. An ensuing mutational analysis identified a highly conserved basic surface patch that binds single-stranded nucleic acids specifically, including the mature CRISPR RNA, but in a sequence-independent manner. In addition, this subunit was found to cleave single-stranded RNA. Together, these studies elucidate the structure and the catalytic activity of the Cmr1 subunit. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | SILENCING COMPLEX | - |
dc.subject | IMMUNE-SYSTEM | - |
dc.subject | CAS SYSTEMS | - |
dc.subject | DNA | - |
dc.subject | PROKARYOTES | - |
dc.subject | REPEATS | - |
dc.subject | CLEAVAGE | - |
dc.subject | RECOGNITION | - |
dc.subject | ELEMENTS | - |
dc.subject | DEFENSE | - |
dc.title | Crystal structure and CRISPR RNA-binding site of the Cmr1 subunit of the Cmr interference complex | - |
dc.type | Article | - |
dc.identifier.wosid | 000331554500032 | - |
dc.identifier.scopusid | 2-s2.0-84894183285 | - |
dc.type.rims | ART | - |
dc.citation.volume | 70 | - |
dc.citation.beginningpage | 535 | - |
dc.citation.endingpage | 543 | - |
dc.citation.publicationname | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | - |
dc.identifier.doi | 10.1107/S1399004713030290 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Sun, Jiali | - |
dc.contributor.nonIdAuthor | Shin, Minsang | - |
dc.contributor.nonIdAuthor | Kim, Jeong-Sun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SILENCING COMPLEX | - |
dc.subject.keywordPlus | IMMUNE-SYSTEM | - |
dc.subject.keywordPlus | CAS SYSTEMS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | PROKARYOTES | - |
dc.subject.keywordPlus | REPEATS | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | ELEMENTS | - |
dc.subject.keywordPlus | DEFENSE | - |
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