Polyplex-releasing microneedles for enhanced cutaneous delivery of DNA vaccine

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dc.contributor.authorKim, Nak Wonko
dc.contributor.authorLee, Min Sangko
dc.contributor.authorKim, Kyu Riko
dc.contributor.authorLee, Jung Eunko
dc.contributor.authorLee, Kyuriko
dc.contributor.authorPark, Jong Sungko
dc.contributor.authorMatsumoto, Yohko
dc.contributor.authorJo, Dong-Gyuko
dc.contributor.authorLee, Haeshinko
dc.contributor.authorLee, Doo Sungko
dc.contributor.authorJeong, Ji Hoonko
dc.date.accessioned2015-01-27T02:03:24Z-
dc.date.available2015-01-27T02:03:24Z-
dc.date.created2014-04-28-
dc.date.created2014-04-28-
dc.date.issued2014-04-
dc.identifier.citationJOURNAL OF CONTROLLED RELEASE, v.179, pp.11 - 17-
dc.identifier.issn0168-3659-
dc.identifier.urihttp://hdl.handle.net/10203/193053-
dc.description.abstractMicroneedle (MN)-based DNA vaccines have many advantages over conventional vaccines administered by hypodermic needles. However, an efficient strategy for delivering DNA vaccines to intradermal cells has not yet been established. Here, we report a new approach for delivering polyplex-based DNA vaccines using MN arrays coated with a pH-responsive polyelectrolyte multilayer assembly (PMA). This approach enabled rapid release of polyplex upon application to the skin. In addition to the polyplex- releasing MNs, we attempted to further maximize the vaccination by developing a polymeric carrier that targeted resident antigen presenting cells (APCs) rich in the intradermal area, as well as a DNA vaccine encoding a secretable fusion protein containing amyloid betamonomer (A beta 1-42), an antigenic determinant. The resulting vaccination systemwas able to successfully induce a robust humoral immune response compared to conventional subcutaneous injection with hypodermal needles. In addition, antigen challenge after immunization elicited an immediate and strong recall immune response due to immunogenic memory. These results suggest the potential utility of MN-based polyplex delivery systems for enhanced DNA vaccination.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectTRANSDERMAL DRUG-DELIVERY-
dc.subjectDENDRITIC CELLS-
dc.subjectBLOCK-COPOLYMERS-
dc.subjectIN-VIVO-
dc.subjectIMMUNIZATION-
dc.subjectINFLUENZA-
dc.subjectSKIN-
dc.subjectNANOPARTICLES-
dc.subjectFORMULATION-
dc.subjectPROTECTION-
dc.titlePolyplex-releasing microneedles for enhanced cutaneous delivery of DNA vaccine-
dc.typeArticle-
dc.identifier.wosid000333249200003-
dc.identifier.scopusid2-s2.0-84893903503-
dc.type.rimsART-
dc.citation.volume179-
dc.citation.beginningpage11-
dc.citation.endingpage17-
dc.citation.publicationnameJOURNAL OF CONTROLLED RELEASE-
dc.identifier.doi10.1016/j.jconrel.2014.01.016-
dc.contributor.localauthorLee, Haeshin-
dc.contributor.nonIdAuthorKim, Nak Won-
dc.contributor.nonIdAuthorLee, Min Sang-
dc.contributor.nonIdAuthorLee, Jung Eun-
dc.contributor.nonIdAuthorLee, Kyuri-
dc.contributor.nonIdAuthorPark, Jong Sung-
dc.contributor.nonIdAuthorMatsumoto, Yoh-
dc.contributor.nonIdAuthorJo, Dong-Gyu-
dc.contributor.nonIdAuthorLee, Doo Sung-
dc.contributor.nonIdAuthorJeong, Ji Hoon-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorDNA vaccine-
dc.subject.keywordAuthorGene delivery-
dc.subject.keywordAuthorMicroneedle array-
dc.subject.keywordAuthorpH-responsive materials-
dc.subject.keywordAuthorLayer-by-layer assembly-
dc.subject.keywordPlusTRANSDERMAL DRUG-DELIVERY-
dc.subject.keywordPlusDENDRITIC CELLS-
dc.subject.keywordPlusBLOCK-COPOLYMERS-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusIMMUNIZATION-
dc.subject.keywordPlusINFLUENZA-
dc.subject.keywordPlusSKIN-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusFORMULATION-
dc.subject.keywordPlusPROTECTION-
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