A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells

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Conventional molecular profiling methods using immunochemical assays have limits in terms of multiplexity and the quantification of biomarkers in investigation of cancer cells. In this paper, we demonstrate a quantum dot (QD)-based microfluidic multiple biomarker quantification (QD-MMBQ) method that enables labeling of more than eight proteins immunochemically on cell blocks within 1 h, in a quantitative manner. An internal reference, beta-actin, was used as a loading control to compensate for differences in not only the cell number but also in staining quality among specimens. Furthermore, the microfluidic blocking method exhibited less nonspecific binding of QDs than the conventional static blocking method.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2014-04
Language
English
Article Type
Article
Keywords

TRASTUZUMAB RESISTANCE; GENE-EXPRESSION; IMMUNOHISTOCHEMISTRY; STANDARDIZATION; DISCOVERY; LINES

Citation

INTEGRATIVE BIOLOGY, v.6, no.4, pp.430 - 437

ISSN
1757-9694
DOI
10.1039/c3ib40224j
URI
http://hdl.handle.net/10203/193016
Appears in Collection
BiS-Journal Papers(저널논문)
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