DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jino | ko |
dc.contributor.author | Kim, Ji Hye | ko |
dc.contributor.author | Lee, Jeewon | ko |
dc.contributor.author | Shin, Sung Jae | ko |
dc.contributor.author | Shin, Eui -Cheol | ko |
dc.date.accessioned | 2014-12-16T01:38:43Z | - |
dc.date.available | 2014-12-16T01:38:43Z | - |
dc.date.created | 2014-07-29 | - |
dc.date.created | 2014-07-29 | - |
dc.date.issued | 2014-07 | - |
dc.identifier.citation | Clinical and experimental vaccine research, v.3, no.2, pp.235 - 243 | - |
dc.identifier.issn | 2287-3651 | - |
dc.identifier.uri | http://hdl.handle.net/10203/192944 | - |
dc.description.abstract | Purpose: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. Materials and Methods: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data. Results: RpfB DNA immunization induced not only humoral immune responses, but also CD8+ and CD4+ T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8+ T cell response that was dominated by a functional phenotype of IFN-γ+/TNF-α+/IL-2-/CD107a+. Conclusion: RpfB DNA immunization elicits polyfunctional CD8+ T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis. | - |
dc.language | English | - |
dc.publisher | 대한백신학회 | - |
dc.title | DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8(+) T cell responses | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.citation.volume | 3 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 235 | - |
dc.citation.endingpage | 243 | - |
dc.citation.publicationname | Clinical and experimental vaccine research | - |
dc.identifier.doi | 10.7774/cevr.2014.3.2.235 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Shin, Eui -Cheol | - |
dc.contributor.nonIdAuthor | Lee, Jeewon | - |
dc.contributor.nonIdAuthor | Shin, Sung Jae | - |
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