DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sangkyu | ko |
dc.contributor.author | Park, Hye Rim | ko |
dc.contributor.author | Kyung, Taeyoon | ko |
dc.contributor.author | Kim, Na Yeon | ko |
dc.contributor.author | Kim, Sungsoo | ko |
dc.contributor.author | Kim, Jihoon | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.date.accessioned | 2014-12-16 | - |
dc.date.available | 2014-12-16 | - |
dc.date.created | 2014-07-07 | - |
dc.date.created | 2014-07-07 | - |
dc.date.created | 2014-07-07 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.citation | NATURE METHODS, v.11, no.6, pp.633 - + | - |
dc.identifier.issn | 1548-7091 | - |
dc.identifier.uri | http://hdl.handle.net/10203/192616 | - |
dc.description.abstract | We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Reversible protein inactivation by optogenetic trapping in cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000336899300020 | - |
dc.identifier.scopusid | 2-s2.0-84901843462 | - |
dc.type.rims | ART | - |
dc.citation.volume | 11 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 633 | - |
dc.citation.endingpage | + | - |
dc.citation.publicationname | NATURE METHODS | - |
dc.identifier.doi | 10.1038/NMETH.2940 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.nonIdAuthor | Lee, Sangkyu | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | GLOBAL ANALYSIS | - |
dc.subject.keywordPlus | BUDDING YEAST | - |
dc.subject.keywordPlus | SMALL GTPASES | - |
dc.subject.keywordPlus | LIVING CELLS | - |
dc.subject.keywordPlus | LIVE CELLS | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | SYSTEM | - |
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