DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hepat, Rahul | ko |
dc.contributor.author | Song, Ji-Joon | ko |
dc.contributor.author | Lee, Daeweon | ko |
dc.contributor.author | Kim, Yonggyun | ko |
dc.date.accessioned | 2014-12-09T01:42:18Z | - |
dc.date.available | 2014-12-09T01:42:18Z | - |
dc.date.created | 2013-11-04 | - |
dc.date.created | 2013-11-04 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.citation | JOURNAL OF VIROLOGY, v.87, no.20, pp.11223 - 11230 | - |
dc.identifier.issn | 0022-538X | - |
dc.identifier.uri | http://hdl.handle.net/10203/192465 | - |
dc.description.abstract | A viral histone H4 (CpBV-H4) is encoded in a polydnavirus, Cotesia plutellae bracovirus. Its predicted amino acid sequence is highly homologous to that of host insect histone H4 except for an extended N-terminal tail containing 38 amino acids with nine lysine residues. Its expression induces an immunosuppression of target insects by suppressing immune-associated genes, presumably through an epigenetic control. This study analyzed its molecular interaction with eukaryotic host nucleosomes and subsequent regulation of host gene expression. Purified recombinant CpBV-H4 could associate with nucleosomal components (H2A, H2B, H3, and H4) and form an octamer. Transient expression of CpBV-H4 in an insect, Tribolium castaneum, was performed by microinjection of a recombinant expression vector and confirmed by both reverse transcriptase PCR (RT-PCR) and immunoblotting assays. Under this transient expression condition, total RNAs were extracted and read by a deep-sequencing technique. Annotated transcripts were classified into different gene ontology (GO) categories and compared with those of control insects injected with a truncated CpBV-H4. Target genes manipulated by CpBV-H4 expression showing significant differences (fold changes > 10(9)) included all GO categories, including development and immune-associated genes. When the target genes were physically mapped, they were found to be scattered on entire chromosomes of T. castaneum. In addition, chromatin immunoprecipitation against CpBV-H4 determined 16 nucleosome sites (P < 10(-5)) of the viral histone incorporation, which were noncoding regions near DNA-binding and inducible genes. These findings suggest that the viral histone H4 alters host gene expression by a direct molecular interaction with insect nucleosomes. | - |
dc.language | English | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | COTESIA-PLUTELLAE BRACOVIRUS | - |
dc.subject | CAMPOLETIS-SONORENSIS | - |
dc.subject | TRANSIENT EXPRESSION | - |
dc.subject | DIAMONDBACK MOTH | - |
dc.subject | HELIOTHIS-VIRESCENS | - |
dc.subject | CHROMATIN-STRUCTURE | - |
dc.subject | POLYDNAVIRUS | - |
dc.subject | GENOME | - |
dc.subject | VIRUS | - |
dc.subject | DNA | - |
dc.title | A Viral Histone H4 Joins to Eukaryotic Nucleosomes and Alters Host Gene Expression | - |
dc.type | Article | - |
dc.identifier.wosid | 000325275800026 | - |
dc.identifier.scopusid | 2-s2.0-84886873485 | - |
dc.type.rims | ART | - |
dc.citation.volume | 87 | - |
dc.citation.issue | 20 | - |
dc.citation.beginningpage | 11223 | - |
dc.citation.endingpage | 11230 | - |
dc.citation.publicationname | JOURNAL OF VIROLOGY | - |
dc.identifier.doi | 10.1128/JVI.01759-13 | - |
dc.contributor.localauthor | Song, Ji-Joon | - |
dc.contributor.nonIdAuthor | Hepat, Rahul | - |
dc.contributor.nonIdAuthor | Lee, Daeweon | - |
dc.contributor.nonIdAuthor | Kim, Yonggyun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | COTESIA-PLUTELLAE BRACOVIRUS | - |
dc.subject.keywordPlus | CAMPOLETIS-SONORENSIS | - |
dc.subject.keywordPlus | TRANSIENT EXPRESSION | - |
dc.subject.keywordPlus | DIAMONDBACK MOTH | - |
dc.subject.keywordPlus | HELIOTHIS-VIRESCENS | - |
dc.subject.keywordPlus | CHROMATIN-STRUCTURE | - |
dc.subject.keywordPlus | POLYDNAVIRUS | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | DNA | - |
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