NTA center dot Ni2+-Functionalized Quantum Dots for VAMP2 Labeling in Live Cells

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An efficient method for labeling individual proteins in live cells is required for investigations into biological mechanisms and cellular processes. Here we describe the preparation of small quantum dots (QDs) that target membrane surface proteins bearing a hexahistidine-tag (His(6)-tag) via specific binding to an nitrilotriacetic acid complex of nickel(II) (NTA center dot Ni2+) on the QD surfaces. We showed that the NTA center dot Ni2+-QDs bound to His-tag functionalized beads as a cellular mimic with high specificity and that QDs successfully targeted His(6)-tagged vesicle-associated membrane proteins (VMAP) on cell surfaces. This strategy provides an efficient approach to monitoring synaptic protein dynamics in spatially restricted and confined biological environments.
Publisher
KOREAN CHEMICAL SOC
Issue Date
2010-06
Language
English
Article Type
Article
Citation

BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.31, no.6, pp.1474 - 1478

ISSN
0253-2964
URI
http://hdl.handle.net/10203/192403
Appears in Collection
BS-Journal Papers(저널논문)
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