This paper reviews our microfluidic approaches for multiplexed immunochemical assays. In particular, topics will be focused on the multiplexed sensing and detection based on the behaviors of immunocomplexed microbeads under magnetophoresis or optoelectrofluidics environment as a new platform for protein sample manipulation and detection. In addition, a microfluidic immunohistochemistry platform will be addressed to give a new insight of biofunctional microfluidics in pathological analysis of breast tissue from patient samples.