DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Seong Min | ko |
dc.contributor.author | Noh, Seung-hyun | ko |
dc.contributor.author | Jin, Zongwen | ko |
dc.contributor.author | Lim, Yongjun | ko |
dc.contributor.author | Cheon, Jinwoo | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2014-09-02T02:31:52Z | - |
dc.date.available | 2014-09-02T02:31:52Z | - |
dc.date.created | 2014-07-04 | - |
dc.date.created | 2014-07-04 | - |
dc.date.issued | 2014-10 | - |
dc.identifier.citation | SENSORS AND ACTUATORS B-CHEMICAL, v.201, pp.144 - 152 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189837 | - |
dc.description.abstract | Capturing tumor cells has attracted the attention because such cells can be used for diagnosing the occurrence and progression of cancers. Here we present a simple and efficient capturing of tumor cells using anti-EGFR antibody-conjugated magnetic nanoparticles. EGFR is a well-established epithelial cancer-specific marker that is over-expressed in many types of tumor cells. The conjugation of an anti-EGFR antibody with magnetic nanoparticles through a PEG-linker was shown to be highly efficient for preventing the non-specific binding of cells and improving the stability of the resulting nanoparticles even at a high salt concentration. The use of EGFR-targetable nanoparticles resulted in about 57% capture efficiency in 20 min for two cancer cell lines, PC9 (lung cancer) and HeLa (cervical cancer). The survival rate of the captured cells was approximately 85%, and most of the cells were retrievable, providing options for a further analysis of captured cells such as a mutational investigation of EGFR-related cancers. The present approach can be used for capturing circulating tumor cells (CTCs) expressing EGFR and the subsequent diagnostic analysis of the captured cells. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.subject | GROWTH-FACTOR RECEPTOR | - |
dc.subject | IRON-OXIDE NANOPARTICLES | - |
dc.subject | LUNG-CANCER | - |
dc.subject | HEPATOCELLULAR-CARCINOMA | - |
dc.subject | ADHESION MOLECULE | - |
dc.subject | WHOLE-BLOOD | - |
dc.subject | BINDING | - |
dc.subject | NANOCRYSTALS | - |
dc.subject | MUTATIONS | - |
dc.subject | THERAPY | - |
dc.title | Simple and efficient capture of EGFR-expressing tumor cells using magnetic nanoparticles | - |
dc.type | Article | - |
dc.identifier.wosid | 000338705700021 | - |
dc.identifier.scopusid | 2-s2.0-84901634780 | - |
dc.type.rims | ART | - |
dc.citation.volume | 201 | - |
dc.citation.beginningpage | 144 | - |
dc.citation.endingpage | 152 | - |
dc.citation.publicationname | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.identifier.doi | 10.1016/j.snb.2014.05.016 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Noh, Seung-hyun | - |
dc.contributor.nonIdAuthor | Lim, Yongjun | - |
dc.contributor.nonIdAuthor | Cheon, Jinwoo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Cancer cell capture | - |
dc.subject.keywordAuthor | Magnetic nanoparticles | - |
dc.subject.keywordAuthor | Epidermal growth factor receptor (EGFR) | - |
dc.subject.keywordAuthor | Immunomagnetic isolation | - |
dc.subject.keywordPlus | GROWTH-FACTOR RECEPTOR | - |
dc.subject.keywordPlus | IRON-OXIDE NANOPARTICLES | - |
dc.subject.keywordPlus | LUNG-CANCER | - |
dc.subject.keywordPlus | HEPATOCELLULAR-CARCINOMA | - |
dc.subject.keywordPlus | ADHESION MOLECULE | - |
dc.subject.keywordPlus | WHOLE-BLOOD | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | NANOCRYSTALS | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | THERAPY | - |
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