Advances in harvesting of microalgae are needed for the efficient and economical production of microalgal biodiesel. In addition to improvements in recovery efficiency, developments in harvest technology should focus on reducing the adverse impact of subsequent processes, and should also allow water recycling. We investigated a continuous electrochemical approach for microalgal biodiesel production. Instead of conventional DC, pulsed DC was applied as a method of polarity exchange and its performance was analyzed in terms of recovery efficiency, electricity consumption, and residual Al concentration. Under optimized pulsed-DC conditions, 32 % less electricity was required and 7 % less Al was remained after continuous harvesting and there was no decrease in recovery efficiency compared to the continuous harvesting by conventional DC. We also examined the effect of this new protocol on biodiesel quality and water reusability. There were no differences in the microalgal oil composition before and after electrolytic harvesting. In addition, the harvested oil quality, based on four key parameters, was superior to that produced by other terrestrial crops. Lastly, there was no retardation of growth in recycled medium relative to that in fresh medium.