DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cui, Chang-Hao | ko |
dc.contributor.author | Kim, Jin-Kwang | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Im, Wan-Taek | ko |
dc.date.accessioned | 2014-09-01T08:35:08Z | - |
dc.date.available | 2014-09-01T08:35:08Z | - |
dc.date.created | 2014-03-03 | - |
dc.date.created | 2014-03-03 | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | PLOS ONE, v.9, no.1 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189584 | - |
dc.description.abstract | A novel beta-glucosidase (BglPm) was identified from Paenibacillus mucilaginosus KCTC 3870(T) which has ginsenoside converting activity. The gene, termed bglPm, consists of 1,260 bp and belongs to glycoside hydrolase family 1 (GH1). After being overexpressed and purified from Escherichia coli, the enzymatic properties of BglPm were investigated. The enzyme exhibited an optimal activity at 45 degrees C and pH 7.5 and showed high bioconversion ability for major ginsenoside Rb-1 and Rd into ginsenoside F-2. Thus, it was used for mass production of relatively high pure F-2 from relatively abundant protopanaxadiol type ginsenosides mixture (PPDGM) with combined usage of ginsenoside Rc-hydrolyzing enzyme. Scale-up of production using 250 g of the PPDGM resulted in 152 g of F-2 with 80.1% chromatography purity and 95.7% recovery. These results suggest that this enzyme would be useful in the preparation of pharmacologically active ginsenoside F-2 in the functional food and pharmaceutical industries. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | ENZYME CHARACTERIZATION | - |
dc.subject | PANAX-GINSENG | - |
dc.subject | BIOCONVERSION | - |
dc.subject | RD | - |
dc.subject | PHARMACOLOGY | - |
dc.subject | NOTOGINSENG | - |
dc.subject | GLYCOSIDASE | - |
dc.subject | EXPRESSION | - |
dc.subject | SEPARATION | - |
dc.subject | CLONING | - |
dc.title | Characterization of a Ginsenoside-Transforming beta-glucosidase from Paenibacillus mucilaginosus and Its Application for Enhanced Production of Minor Ginsenoside F-2 | - |
dc.type | Article | - |
dc.identifier.wosid | 000330507300023 | - |
dc.identifier.scopusid | 2-s2.0-84899963209 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 1 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0085727 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Kim, Jin-Kwang | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ENZYME CHARACTERIZATION | - |
dc.subject.keywordPlus | PANAX-GINSENG | - |
dc.subject.keywordPlus | BIOCONVERSION | - |
dc.subject.keywordPlus | RD | - |
dc.subject.keywordPlus | PHARMACOLOGY | - |
dc.subject.keywordPlus | NOTOGINSENG | - |
dc.subject.keywordPlus | GLYCOSIDASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | CLONING | - |
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