Highly efficient colorimetric detection of target cancer cells utilizing superior catalytic activity of graphene oxide-magnetic-platinum nanohybrids

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Enzyme-linked immunosorbent assays (ELISAs) have most widely been applied in immunoassays for several decades. However, several unavoidable limitations (e.g., instability caused by structural unfolding) of natural enzymes have hindered their widespread applications. Here, we describe a new nanohybrid consisting of Fe3O4 magnetic nanoparticles (MNPs) and platinum nanoparticles (Pt NPs), simultaneously immobilized on the surface of graphene oxide (GO). By synergistically integrating highly catalytically active Pt NPs and MNPs on GO whose frameworks possess high substrate affinity, the nanohybrid is able to achieve up to a 30-fold higher maximal reaction velocity (V-max) compared to that of free GO for the colorimetric reaction of the peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB), and enable rapid detection of target cancer cells. Specifically, using this new assay system, clinically important breast cancer cells are detected in a 5 min time period at room temperature with high specificity and sensitivity. The remarkably high capability to catalyze oxidation reactions could allow the nanohybrid to replace conventional peroxidase-based immunoassay systems as part of new, rapid, robust and convenient assay systems which can be widely utilized for the identification of important target molecules.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2014-02
Language
English
Article Type
Article
Keywords

METASTATIC BREAST-CANCER; GROWTH-FACTOR RECEPTOR; IN-SITU HYBRIDIZATION; ELECTROCHEMICAL DETECTION; FUNCTIONALIZED GRAPHENE; BIOSENSING PLATFORM; NANOPARTICLES; AMPLIFICATION; BIOMOLECULES; DEPOSITION

Citation

NANOSCALE, v.6, no.3, pp.1529 - 1536

ISSN
2040-3364
DOI
10.1039/c3nr05539f
URI
http://hdl.handle.net/10203/189447
Appears in Collection
CBE-Journal Papers(저널논문)
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