DC Field | Value | Language |
---|---|---|
dc.contributor.author | Du, Juan | ko |
dc.contributor.author | CUI, CHANGHAO | ko |
dc.contributor.author | Park, Sung Chul | ko |
dc.contributor.author | Kim, Jin-Kwang | ko |
dc.contributor.author | Yu, Hong-Shan | ko |
dc.contributor.author | Jin, Feng-Xie | ko |
dc.contributor.author | Sun, Changkai | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Im, Wan-Taek | ko |
dc.date.accessioned | 2014-09-01T08:08:22Z | - |
dc.date.available | 2014-09-01T08:08:22Z | - |
dc.date.created | 2014-07-17 | - |
dc.date.created | 2014-07-17 | - |
dc.date.issued | 2014-06 | - |
dc.identifier.citation | PLOS ONE, v.9, no.6 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/189407 | - |
dc.description.abstract | The ginsenoside Rg(2)(S), which is one of the pharmaceutical components of ginseng, is known to have neuroprotective, antiinflammation, and anti-diabetic effects. However, the usage of ginsenoside Rg2(S) is restricted owing to the small amounts found in white and red ginseng. To enhance the production of ginsenoside Rg2(S) as a 100 gram unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the recombinant glycoside hydrolase (BglPC28), which is a ginsenoside-transforming recombinant beta-glucosidase from Pseudonocardia sp. strain Gsoil 1536. The gene, termed bglPC28, encoding beta-glucosidase (BglPC28) belonging to the glycoside hydrolase family 3 was cloned. bglPC28 consists of 2,232 bp (743 amino acid residues) with a predicted molecular mass of 78,975 Da. This enzyme was overexpressed in Escherichia coli BL21(DE3) using a GST-fused pGEX 4T-1 vector system. The optimum conditions of the recombinant BglPC28 were pH 7.0 and 37 degrees C. BglPC28 can effectively transform the ginsenoside Re to Rg2(S); the Km values of PNPG and Re were 6.36 +/- 1.10 and 1.42 +/- 0.13 mM, respectively, and the V-max values were 40.0 +/- 2.55 and 5.62 +/- 0.21 mmol min(-1) mg(-)1 of protein, respectively. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 7.0 and 30 degrees C for 12 hours with a concentration of 20 mg/ml of ginsenoside Re from American ginseng roots. Finally, 113 g of Rg2(S) was produced from 150 g of Re with 84.0 +/- 1.1% chromatographic purity. These results suggest that this enzymatic method could be usefully exploited in the preparation of ginsenoside Rg(2)(S) in the cosmetics, functional food, and pharmaceutical industries. | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | ALPHA-L-ARABINOFURANOSIDASE | - |
dc.subject | PANAX-GINSENG | - |
dc.subject | COMPOUND K | - |
dc.subject | ENZYME CHARACTERIZATION | - |
dc.subject | MICROBACTERIUM-ESTERAROMATICUM | - |
dc.subject | SIMULTANEOUS QUANTIFICATION | - |
dc.subject | GLYCOSIDE HYDROLASES | - |
dc.subject | CONVERTING ACTIVITY | - |
dc.subject | RARE GINSENOSIDES | - |
dc.subject | PA14 DOMAIN | - |
dc.title | Identification and Characterization of a Ginsenoside-Transforming beta-Glucosidase from Pseudonocardia sp Gsoil 1536 and Its Application for Enhanced Production of Minor Ginsenoside Rg(2)(S) | - |
dc.type | Article | - |
dc.identifier.wosid | 000337165600123 | - |
dc.identifier.scopusid | 2-s2.0-84902665729 | - |
dc.type.rims | ART | - |
dc.citation.volume | 9 | - |
dc.citation.issue | 6 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0096914 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Du, Juan | - |
dc.contributor.nonIdAuthor | Park, Sung Chul | - |
dc.contributor.nonIdAuthor | Kim, Jin-Kwang | - |
dc.contributor.nonIdAuthor | Yu, Hong-Shan | - |
dc.contributor.nonIdAuthor | Jin, Feng-Xie | - |
dc.contributor.nonIdAuthor | Sun, Changkai | - |
dc.contributor.nonIdAuthor | Im, Wan-Taek | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ALPHA-L-ARABINOFURANOSIDASE | - |
dc.subject.keywordPlus | PANAX-GINSENG | - |
dc.subject.keywordPlus | COMPOUND K | - |
dc.subject.keywordPlus | ENZYME CHARACTERIZATION | - |
dc.subject.keywordPlus | MICROBACTERIUM-ESTERAROMATICUM | - |
dc.subject.keywordPlus | SIMULTANEOUS QUANTIFICATION | - |
dc.subject.keywordPlus | GLYCOSIDE HYDROLASES | - |
dc.subject.keywordPlus | CONVERTING ACTIVITY | - |
dc.subject.keywordPlus | RARE GINSENOSIDES | - |
dc.subject.keywordPlus | PA14 DOMAIN | - |
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