Variable allelic expression of imprinted genes in human pluripotent stem cells during differentiation into specialized cell types in vitro

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Genomic imprinting is an epigenetic phenomenon by which a subset of genes is asymmetrically expressed in a parent-of-origin manner. However, little is known regarding the epigenetic behaviors of imprinted genes during human development. Here, we show dynamic epigenetic changes in imprinted genes in hESCs during in vitro differentiation into specialized cell types. Out of 9 imprinted genes with single nucleotide polymorphisms, mono-allelic expression for three imprinted genes (H19, KCNQ10T1, and IPW), and bi- or partial-allelic expression for three imprinted genes (OSBPL5, PPP1R9A, and RTL1) were stably retained in H9-hESCs throughout differentiation, representing imprinting stability. Three imprinted genes (KCNK9, ATP10A, and SLC22A3) showed a loss and a gain of imprinting in a lineage-specific manner during differentiation. Changes in allelic expression of imprinted genes were observed in another hESC line during in vitro differentiation. These findings indicate that the allelic expression of imprinted genes may be vulnerable in a lineage-specific manner in human pluripotent stem cells during differentiation.
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Issue Date
2014-04
Language
English
Article Type
Article
Keywords

DNA METHYLATION; GENOME; MOUSE; CLUSTERS; LINES

Citation

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.446, no.2, pp.493 - 498

ISSN
0006-291X
DOI
10.1016/j.bbrc.2014.02.141
URI
http://hdl.handle.net/10203/189062
Appears in Collection
BS-Journal Papers(저널논문)
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