DC Field | Value | Language |
---|---|---|
dc.contributor.author | Min, Junhong | ko |
dc.contributor.author | Vonesch, Cedric | ko |
dc.contributor.author | Kirshner, Hagai | ko |
dc.contributor.author | Carlini, Lina | ko |
dc.contributor.author | Olivier, Nicolas | ko |
dc.contributor.author | Holden, Seamus | ko |
dc.contributor.author | Manley, Suliana | ko |
dc.contributor.author | Ye, Jong Chul | ko |
dc.contributor.author | Unser, Michael | ko |
dc.date.accessioned | 2014-08-29T02:03:11Z | - |
dc.date.available | 2014-08-29T02:03:11Z | - |
dc.date.created | 2014-05-13 | - |
dc.date.created | 2014-05-13 | - |
dc.date.created | 2014-05-13 | - |
dc.date.created | 2014-05-13 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v.4 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10203/188937 | - |
dc.description.abstract | Super resolution microscopy such as STORM and (F) PALM is now a well known method for biological studies at the nanometer scale. However, conventional imaging schemes based on sparse activation of photo-switchable fluorescent probes have inherently slow temporal resolution which is a serious limitation when investigating live-cell dynamics. Here, we present an algorithm for high-density super-resolution microscopy which combines a sparsity-promoting formulation with a Taylor series approximation of the PSF. Our algorithm is designed to provide unbiased localization on continuous space and high recall rates for high-density imaging, and to have orders-of-magnitude shorter run times compared to previous high-density algorithms. We validated our algorithm on both simulated and experimental data, and demonstrated live-cell imaging with temporal resolution of 2.5 seconds by recovering fast ER dynamics. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | LOCALIZATION MICROSCOPY | - |
dc.subject | LIVE CELLS | - |
dc.subject | STORM | - |
dc.subject | RESOLUTION | - |
dc.subject | NANOSCOPY | - |
dc.title | FALCON: fast and unbiased reconstruction of high-density super-resolution microscopy data | - |
dc.type | Article | - |
dc.identifier.wosid | 000333797300004 | - |
dc.identifier.scopusid | 2-s2.0-84897513305 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.publicationname | SCIENTIFIC REPORTS | - |
dc.identifier.doi | 10.1038/srep04577 | - |
dc.contributor.localauthor | Ye, Jong Chul | - |
dc.contributor.nonIdAuthor | Vonesch, Cedric | - |
dc.contributor.nonIdAuthor | Kirshner, Hagai | - |
dc.contributor.nonIdAuthor | Carlini, Lina | - |
dc.contributor.nonIdAuthor | Olivier, Nicolas | - |
dc.contributor.nonIdAuthor | Holden, Seamus | - |
dc.contributor.nonIdAuthor | Manley, Suliana | - |
dc.contributor.nonIdAuthor | Unser, Michael | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | LOCALIZATION MICROSCOPY | - |
dc.subject.keywordPlus | LIVE CELLS | - |
dc.subject.keywordPlus | STORM | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | NANOSCOPY | - |
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