DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Se Hyeuk | ko |
dc.contributor.author | Lee, Jung Min | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Park, Chan Bae | ko |
dc.contributor.author | Lee, Pyung Cheon | ko |
dc.date.accessioned | 2014-08-29T02:03:09Z | - |
dc.date.available | 2014-08-29T02:03:09Z | - |
dc.date.created | 2014-05-13 | - |
dc.date.created | 2014-05-13 | - |
dc.date.issued | 2014-04 | - |
dc.identifier.citation | BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, v.92, no.2, pp.105 - 111 | - |
dc.identifier.issn | 0829-8211 | - |
dc.identifier.uri | http://hdl.handle.net/10203/188936 | - |
dc.description.abstract | We investigated the cytotoxic activities of crocin and crocetin, 2 major carotenoids isolated from the stigma of Crocus sativus ( saffron), on 5 human cancer cell lines and proposed their possible anticancer mechanisms. Crocetin, a glycosylated carotenoid, showed approximately 5- to 18-fold higher cytotoxicity than crocin, a carboxylic carotenoid (IC50 of 0.16-0.61 mmol/L for crocetin vs. 2.0-5.5 mmol/L for crocin). This suggests that structural differences account for the different efficacies between them. Fluorescence-activated cell sorting (FACS) analysis showed that crocetin induced a significant level of cellular reactive oxygen species (ROS) in HeLa cells, whereas crocin did not. This ROS induction supported the cytotoxicity of crocetin, but not of crocin. A significant activation of nuclear factor erythroid 2-related factor 2 (Nrf2) was observed in both HeLa cells treated with crocin and crocetin: a 3.0-fold increase by 1 mmol/L crocetin and a 1.6-fold increase by 0.8 mmol/L crocin compared to the control. Furthermore, both crocetin and crocin reduced the protein expression of lactate dehydrogenase A (LDHA), one of the targets for chemoprevention in cancer cells, by 34.2% and 10.5%, respectively, compared to the control in HeLa cells. These findings suggest that crocetin and crocin have different mechanisms for their observed cytotoxicity in cancer cell lines. | - |
dc.language | English | - |
dc.publisher | CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS | - |
dc.subject | ELEMENT TRANSCRIPTION SYSTEM | - |
dc.subject | APOPTOGENIC PROPERTIES | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | IN-VITRO | - |
dc.subject | NRF2 | - |
dc.subject | DIMETHYLCROCETIN | - |
dc.subject | ATTENUATION | - |
dc.subject | EXPRESSION | - |
dc.subject | GROWTH | - |
dc.title | Proposed cytotoxic mechanisms of the saffron carotenoids crocin and crocetin on cancer cell lines | - |
dc.type | Article | - |
dc.identifier.wosid | 000334029200003 | - |
dc.identifier.scopusid | 2-s2.0-84898070696 | - |
dc.type.rims | ART | - |
dc.citation.volume | 92 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 105 | - |
dc.citation.endingpage | 111 | - |
dc.citation.publicationname | BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | - |
dc.identifier.doi | 10.1139/bcb-2013-0091 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Kim, Se Hyeuk | - |
dc.contributor.nonIdAuthor | Lee, Jung Min | - |
dc.contributor.nonIdAuthor | Park, Chan Bae | - |
dc.contributor.nonIdAuthor | Lee, Pyung Cheon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | crocin | - |
dc.subject.keywordAuthor | crocetin | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | LDHA | - |
dc.subject.keywordPlus | ELEMENT TRANSCRIPTION SYSTEM | - |
dc.subject.keywordPlus | APOPTOGENIC PROPERTIES | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordPlus | DIMETHYLCROCETIN | - |
dc.subject.keywordPlus | ATTENUATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GROWTH | - |
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