DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seung Chul Jun | ko |
dc.contributor.author | Lee, Gyun Min | ko |
dc.contributor.author | Su Hwan Chang | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.date.accessioned | 2010-05-14T06:06:44Z | - |
dc.date.available | 2010-05-14T06:06:44Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1996 | - |
dc.identifier.citation | CYTOTECHNOLOGY, v.19, no.2, pp.153 - 159 | - |
dc.identifier.issn | 0920-9069 | - |
dc.identifier.uri | http://hdl.handle.net/10203/18328 | - |
dc.description.abstract | The effect of serum type and concentration on recombinant protein expression in vaccinia virus infected HeLa S3 cells was studied in both static and suspension culture. A model heterologous protein, β- galactosidase (β-gal), was used. Calf and horse sera in the range of 0.5- 10%(v/v) were investigated. In static culture, the calf serum concentration did not show any significant influence on the β-gal production which was almost completed within 24 h postinfection (pi). Higher horse serum concentration, on the other hand, resulted in higher β-gal concentration which continued to increase until 48 h pi. Total β-gal concentrations in 0.5% calf serum at 24 h pi and 10% horse serum at 48 h pi were 2.2 ± 0.7 and 2.2 ± 0.1 IU/ml, respectively. In suspension culture, both sera showed their respective effects on the β-gal production similar to those observed in static culture, indicating that the cultivation method had little influence on β-gal production. Accordingly, the use of 0.5% calf serum after virus infection is recommended for economical β-gal production. | - |
dc.description.sponsorship | This work was supported by the KOSEF research grant to the BERC in KAIST. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Springer Netherlands | - |
dc.subject | ANIMAL-CELLS | - |
dc.subject | ADSORPTION | - |
dc.subject | PROTEINS | - |
dc.subject | SYSTEM | - |
dc.subject | GROWTH | - |
dc.subject | ENTRY | - |
dc.subject | TOOL | - |
dc.title | Effect of serum type and concentration on the expression of beta-galactosidase in recombinant vaccinia virus infected HeLa S3 cells | - |
dc.type | Article | - |
dc.identifier.wosid | A1996VC93100007 | - |
dc.identifier.scopusid | 2-s2.0-0029548455 | - |
dc.type.rims | ART | - |
dc.citation.volume | 19 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 153 | - |
dc.citation.endingpage | 159 | - |
dc.citation.publicationname | CYTOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/BF00749770 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Gyun Min | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | Seung Chul Jun | - |
dc.contributor.nonIdAuthor | Su Hwan Chang | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | vaccinia virus | - |
dc.subject.keywordAuthor | HeLa S3 cells | - |
dc.subject.keywordAuthor | beta-galactosidase | - |
dc.subject.keywordAuthor | calf and horse serum | - |
dc.subject.keywordPlus | ANIMAL-CELLS | - |
dc.subject.keywordPlus | ADSORPTION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | ENTRY | - |
dc.subject.keywordPlus | TOOL | - |
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