DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeong, Yeon Tae | ko |
dc.contributor.author | Choi, One | ko |
dc.contributor.author | Son, Young Dok | ko |
dc.contributor.author | Park, Seung Yeol | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.date.accessioned | 2010-05-14T02:19:19Z | - |
dc.date.available | 2010-05-14T02:19:19Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, v.52, no.4, pp.283 - 291 | - |
dc.identifier.issn | 0885-4513 | - |
dc.identifier.uri | http://hdl.handle.net/10203/18320 | - |
dc.description.abstract | Sialic acid, the terminal sugar in N-linked complex glycans, is usually found in glycoproteins and plays a major role in determining the circulatory lifespan of glycoproteins. In the present study we attempted to enhance the sialylation of recombinant EPO (erythropoietin) in CHO (Chinese-hamster ovary) cells. To enhance EPO sialylation, we introduced human alpha 2,3-ST (alpha 2,3-sialyltransferase) and CMP-SAS (CMP-sialic acid synthase) into recombinant human EPO-producing CHO cells. The sialylation of EPO was increased by the expression of alpha 2,3-ST alone. Although the co-expression of alpha 2,3-ST and CMP-SAS did not further increase sialylation, an increase in the intracellular pool of CMP-sialic acid was noted. On the basis of these observations, it was postulated that the transport capacity of CMP-sialic acid into the Golgi lumen was limited, thereby causing the reduced availability of CMP-sialic acid substrate for sialylation. Therefore, we co-expressed human alpha 2,3-ST and CMP-SAS, as well as overexpress Chinese hamster CMP-sialic acid transporter (CMP-SAT) in CHO cells, which produced recombinant human EPO. When alpha 2,3-ST, CMP-SAS, and CMP-SAT were overexpressed in CHO cells, there was a corresponding increase in siallylation compared with the co-expression of alpha 2,3-ST and CMP-SAS. The present study provides a useful strategy for enhancing the sialylation of therapeutic glycoproteins produced in CHO cells. | - |
dc.description.sponsorship | This work was supported by the Korea Science and Engineering Foundation [grant no. M10619000005-07N1900-00511]. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | PORTLAND PRESS LTD | - |
dc.subject | CMP-SIALIC ACID | - |
dc.subject | ACETYLGLUCOSAMINE 2-EPIMERASE/N-ACETYLMANNOSAMINE KINASE | - |
dc.subject | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject | N-ACETYLNEURAMINIC ACID | - |
dc.subject | INTERFERON-GAMMA | - |
dc.subject | INSECT CELLS | - |
dc.subject | CHO-CELLS | - |
dc.subject | METABOLIC CONTROL | - |
dc.subject | SUGAR CHAINS | - |
dc.subject | GLYCOSYLATION | - |
dc.title | Enhanced sialylation of recombinant erythropoietin in genetically engineered Chinese-hamster ovary cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000264903400004 | - |
dc.identifier.scopusid | 2-s2.0-66149104098 | - |
dc.type.rims | ART | - |
dc.citation.volume | 52 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 283 | - |
dc.citation.endingpage | 291 | - |
dc.citation.publicationname | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | - |
dc.identifier.doi | 10.1042/BA20080044 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | Jeong, Yeon Tae | - |
dc.contributor.nonIdAuthor | Choi, One | - |
dc.contributor.nonIdAuthor | Son, Young Dok | - |
dc.contributor.nonIdAuthor | Park, Seung Yeol | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Chinese-hamster ovary cells (CHO cells) | - |
dc.subject.keywordAuthor | CIMP-sialic acid synthase | - |
dc.subject.keywordAuthor | CMP-sialic acid transporter | - |
dc.subject.keywordAuthor | erythropoietin | - |
dc.subject.keywordAuthor | sialylation | - |
dc.subject.keywordAuthor | sialyltransferase | - |
dc.subject.keywordPlus | CMP-SIALIC ACID | - |
dc.subject.keywordPlus | ACETYLGLUCOSAMINE 2-EPIMERASE/N-ACETYLMANNOSAMINE KINASE | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | N-ACETYLNEURAMINIC ACID | - |
dc.subject.keywordPlus | INTERFERON-GAMMA | - |
dc.subject.keywordPlus | INSECT CELLS | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | METABOLIC CONTROL | - |
dc.subject.keywordPlus | SUGAR CHAINS | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
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