DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Dong-Yoon | ko |
dc.contributor.author | Kim, Young-Pil | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.contributor.author | Ko Park, Sang-Hee | ko |
dc.contributor.author | Choi, Sung-Yool | ko |
dc.contributor.author | Choi, Yang-Kyu | ko |
dc.date.accessioned | 2010-05-10T02:31:09Z | - |
dc.date.available | 2010-05-10T02:31:09Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007-03 | - |
dc.identifier.citation | JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B, v.25, no.2, pp.443 - 447 | - |
dc.identifier.issn | 1071-1023 | - |
dc.identifier.uri | http://hdl.handle.net/10203/18145 | - |
dc.description.abstract | Label-free electrical detection of protein-ligand binding using a vertical gold nanogap is presented. A sublithographic nanogap was created using a sacrificial ultrathin film deposited by atomic layer deposition (ALD) in a process similar to the formation of a cantilever. in microelectromechanical system processing. Due to the atomic precision of the sacrificial Al2O3 thickness by ALD, a 7 nm nanogap was successfully fabricated. After binding streptavidin to biotin on the gold surface, an electrical current was measured for various voltages. A dramatic current increase was observed in the case of biotin-streptavidin binding in comparison with the other two cases: a control group filled with air and a biotin-only binding group. There was a minimal current change in the cases of the biotin-PBST group, the biotin-BSA group, and the biotin-saturated streptavidin group, as compared with the biotin-streptavidin group. At a 0.1 mu g/ml concentration of streptavidin (1.5 nM), the current difference before and after the protein binding was amplified by approximately 3000-fold with 17 nm nanogap. Also, the detection sensitivity of the vertical nanogap as the gap size varied was investigated. As the size of biotin-streptavidin binding is the most comparable to 12 nm nanogap, the highest sensitivity was shown in the 12 nm gap device. 7 nm nanogap can be used to detect smaller size of biomolecule than that of biotin streptavidin. This arrayable, two-terminal microdevice could be tested for use on a wide range of other biomolecules. (c) 2007 American Vacuum Society. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | A V S AMER INST PHYSICS | - |
dc.title | Sublithographic vertical gold nanogap for label-free electrical detection of protein-ligand binding | - |
dc.type | Article | - |
dc.identifier.wosid | 000246045300026 | - |
dc.identifier.scopusid | 2-s2.0-34047144477 | - |
dc.type.rims | ART | - |
dc.citation.volume | 25 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 443 | - |
dc.citation.endingpage | 447 | - |
dc.citation.publicationname | JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B | - |
dc.identifier.doi | 10.1116/1.2713403 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Ko Park, Sang-Hee | - |
dc.contributor.localauthor | Choi, Sung-Yool | - |
dc.contributor.localauthor | Choi, Yang-Kyu | - |
dc.contributor.nonIdAuthor | Jang, Dong-Yoon | - |
dc.contributor.nonIdAuthor | Kim, Young-Pil | - |
dc.contributor.nonIdAuthor | Kim, Hak-Sung | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | BIOSENSOR | - |
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