DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Eun-Kyung | ko |
dc.contributor.author | Lee, Bong-Soo | ko |
dc.contributor.author | Pyo, Hyeon-Bong | ko |
dc.contributor.author | Song, Hyun-Woo | ko |
dc.contributor.author | Kim, Young-Pil | ko |
dc.contributor.author | Choi, In-Sung | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2010-05-10T02:03:24Z | - |
dc.date.available | 2010-05-10T02:03:24Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2008-06 | - |
dc.identifier.citation | BIOCHIP JOURNAL, v.2, no.2, pp.103 - 110 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | http://hdl.handle.net/10203/18140 | - |
dc.description.abstract | Reducing the non-specific binding of proteins on a chip surface is essential for the development of biosensors with high sensitivity and specificity. We fabricated poly(oligo(ethylene glycol) methacrylate) (pOEGMA) films with various thicknesses as a nonbiofouling surface for surface plasmon field-enhanced fluorescence spectroscopy (Sill To accomplish a sensitive SPF signal, the thickness of the pOEGMA films was controlled by surface-initiated atom transfer radical polymerization (SI-ATRP) on gold-coated glass (Au/Glass) and gold-coated Si (Au/Si) substrates. When fluorophores immobilized on the pOEGMA films were directly excited without a plasmon effect, the fluorescence intensity displayed a linear dependency on the tested thickness of the pOEGMA films on both Au/Si (9-60 nm) and Au/Glass (8-56 nm). As a result, the surface plasmon field-induced fluorescence intensity on Au/Gilass enabled thickness-dependent sensitivity. The Sill signal on 50-nm-thick pOEGMA films was estimated to be 100-fold stronger than that on 13-nm-thick pOEGMA films. Since the SPFS on pOEGMA film can allow an easy conjugation of biomolecules and provide a high resistance to biofouling events, our fabricated system has a great potential as a platform for biosensors with high sensitivity. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | KOREAN BIOCHIP SOC | - |
dc.subject | RESONANCE | - |
dc.subject | FUNCTIONALIZATION | - |
dc.subject | DISTANCE | - |
dc.subject | FILMS | - |
dc.subject | CELLS | - |
dc.subject | GOLD | - |
dc.title | Fabrication of nonbiofouling surface and its application to surface plasmon field-enhanced fluorescence spectroscopy | - |
dc.type | Article | - |
dc.identifier.wosid | 000258694400003 | - |
dc.identifier.scopusid | 2-s2.0-70449097455 | - |
dc.type.rims | ART | - |
dc.citation.volume | 2 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 103 | - |
dc.citation.endingpage | 110 | - |
dc.citation.publicationname | BIOCHIP JOURNAL | - |
dc.contributor.localauthor | Choi, In-Sung | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.nonIdAuthor | Pyo, Hyeon-Bong | - |
dc.contributor.nonIdAuthor | Song, Hyun-Woo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | nonbiofouling surface | - |
dc.subject.keywordAuthor | poly(oligo(ethyleneglycol) methacrylate) films | - |
dc.subject.keywordAuthor | surface plasmon field-enhanced fluorescence spectroscopy | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordPlus | RESONANCE | - |
dc.subject.keywordPlus | FUNCTIONALIZATION | - |
dc.subject.keywordPlus | DISTANCE | - |
dc.subject.keywordPlus | FILMS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | GOLD | - |
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