A label-free DNA detection method utilizing capacitive touchscreen = 정전용량 방식 터치스크린을 이용한 비표지 핵산 검출 방법 개발

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A touchscreen is an electronic visual display that can detect the presence and location of a touch within the display area. It is generally used in personal digital assistants (PDAs), satellite navigation devices, and smart phones. There are some kind of touchscreen technology according to methods of sensing touch, which are resistive, infrared, surface acoustic wave (SAW), and capacitive touchscreen. Among them, the capacitive touchscreen has been widely used recently because of increasing smart phone users. Basically, touching the surface of the screen induces capacitance changes of electrodes which can be detected by a capacitive touchscreen. In this study, a novel label-free DNA detection system was developed based on the capacitive property of DNA which could be detected by the capacitive touchscreen. For this purpose, we first fabricated the touchscreen panel by patterning the ITO electrodes on the glass panel, and the ITO electrodes were connected to the capacitive touchscreen controller. On the touchscreen panel, the PDMS chamber was covered and ITO electrodes were chemically modified by 3-aminopropyltrimethoxysilane (APTES). For covalent binding of capture probes after spotting, the electrodes were exposed to UV light for 10 min. The capture probe was specifically designed to be complementary to the target pathogen, Chlamydia trachomatis DNA. The capture probe-modified electrodes were rinsed with water and Tris buffer before target addition. To detect target DNA, we applied DNA samples to the capture probe immobilized ITO electrodes and washed with SSPE buffer after 30 min hybridization. In the case of the target DNA in the samples, the capacitance signal of ITO layer increased. On the other hand, when there is no target DNA in the sample, no capacitance change was recorded. With this system, we have successfully detected target DNA in a label-free manner. This new label-free DNA detection strategy has some advantages owing to properties of touchscree...
Advisors
Park, Hyun-Gyuresearcher박현규Seo, Tae-Seok서태석
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2013
Identifier
515050/325007  / 020113481
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2013.2, [ vii, 32 p. ]

Keywords

DNA detection; touchscreen; electrochemical biosensor; POCT; 핵산 검출; 터치스크린; 전기화학 바이오센서; 정전용량 바이오센서; 현장진단; capacitive biosensor

URI
http://hdl.handle.net/10203/179983
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=515050&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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