High-throughput Synthesis of Multi-functional Nanoscale Lipid Vesicles in a Contraction??Expansion Array Microchannel = 확장-축소 어레이 구조를 갖는 마이크로채널 내에서 다기능 나노 지질소포체의 고속 합성

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 362
  • Download : 0
This paper reports a simple method of encapsulating materials inside of lipid vesicles, along with tunable size and enhanced polydispersity of the size distribution. We fabricated a contraction??expansion array (CEA) microchannel in the shape of semicircular groove arrays by using poly(dimethylsiloxane) molding technique. The device has the 50 ??m deep microchannel containing a uniform array of contraction??expansion microchannel. The channel has a width of 350 ??m and 50 ??m on the expansion and contraction region, respectively. The interval between each region is 600 ??m. The entire mixing channel consists of 30 array units. Lipid vesicles were generated in the CEA microchannel by injecting lipids??dimyristoylphosphatidylcholine and cholesterol in a molar ratio of 1:1??in isopropyl alcohol as a sample flow and phosphate buffered saline (PBS) as a buffer flow, leading to spontaneous formation of liposomes. In the CEA microchannel, the fluid experiences Dean vortices at high Re (> 5), passing through the contraction regions. The injected lipids are rapidly mixed with PBS by three-dimensional (3D) laminating effect attributed from the Dean vortices. In such a CEA microchannel, Dean vortices lead to 3D lamination effected by continuously splitting and redirecting fluid streams, resulting in enhancement of fluid mixing. In addition, the injected lipids and PBS experience high shear stress relative to reducing size distribution of lipid vesicles throughout the CEA microchannel. From the interplay between high shear stress and 3D laminating effect, the lipid vesicles are generated with monodispersity and high throughput. Consequently, the formation of lipid vesicles can be controlled with a total flow rate and a flow rate ratio between the sample and buffer fluids. Generated lipid vesicles from the CEA microchannel showed narrower size distribution than ones from the linear microchannel at a flow rate of 18 mL/h (Re = ~25). The throughput of the lipid generation in...
Advisors
Park, Je-Kyunresearcher박제균
Description
한국과학기술원 : 바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2011
Identifier
482746/325007  / 020094250
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2011.8, [ v, 40 p. ]

Keywords

확장-축소 어레이 구조; 합성; 단순분산; 리포솜; Contraction??expansion array microchannel; Synthesis; monodispersity; Liposome; High-throughput; 고처리량

URI
http://hdl.handle.net/10203/179760
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=482746&flag=dissertation
Appears in Collection
BiS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0