DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ji-Hyo | ko |
dc.contributor.author | Seo, Moon-Hyeong | ko |
dc.contributor.author | Lee, Sang-Sik | ko |
dc.contributor.author | Cho, Kyu-Kwang | ko |
dc.contributor.author | Yang, Ae-Rin | ko |
dc.contributor.author | Woo, Kyung-Hwa | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.contributor.author | Park, Hee-Sung | ko |
dc.date.accessioned | 2013-08-08T01:54:16Z | - |
dc.date.available | 2013-08-08T01:54:16Z | - |
dc.date.created | 2013-03-18 | - |
dc.date.created | 2013-03-18 | - |
dc.date.issued | 2013-02 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, v.85, no.3, pp.1468 - 1474 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://hdl.handle.net/10203/174172 | - |
dc.description.abstract | Analysis of protein dynamics using single-molecule fluorescence resonance energy transfer (smFRET) is widely used to understand the structure and function of proteins. Nonetheless, site-specific labeling of proteins with a pair of donor and acceptor dyes still remains a challenge. Here we present a general and facile method for site-specific dual labeling of proteins by incorporating two different, readily available, unnatural amino acids (p-acetylphenylalanine and alkynyllysine) for smFRET. We used newly evolved alkynyllysine-specific aminoacyl-tRNA synthetase/tRNA(UCA) and p-acetylphenylalanyl-tRNA synthetase/tRNA(CUA). The utility of our approach was demonstrated by analyzing the conformational change of dual-labeled calmodulin using smFRET measurements. The present labeling approach is devoid of major limitations in conventional cysteine-based labeling. Therefore, our method will significantly increase the repertoire of proteins available for FRET study and expand our ability to explore more complicated molecular dynamics. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | NONCANONICAL AMINO-ACIDS | - |
dc.subject | TRANSFER-RNA SYNTHETASE | - |
dc.subject | GENETIC-CODE | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | CONFORMATIONAL STATES | - |
dc.subject | RIBOSOME | - |
dc.subject | ENZYME | - |
dc.subject | FRET | - |
dc.title | Simple and Efficient Strategy for Site-Specific Dual Labeling of Proteins for Single-Molecule Fluorescence Resonance Energy Transfer Analysis | - |
dc.type | Article | - |
dc.identifier.wosid | 000314676100035 | - |
dc.identifier.scopusid | 2-s2.0-84873380179 | - |
dc.type.rims | ART | - |
dc.citation.volume | 85 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 1468 | - |
dc.citation.endingpage | 1474 | - |
dc.citation.publicationname | ANALYTICAL CHEMISTRY | - |
dc.identifier.doi | 10.1021/ac303089v | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.contributor.localauthor | Park, Hee-Sung | - |
dc.contributor.nonIdAuthor | Kim, Ji-Hyo | - |
dc.contributor.nonIdAuthor | Lee, Sang-Sik | - |
dc.contributor.nonIdAuthor | Cho, Kyu-Kwang | - |
dc.contributor.nonIdAuthor | Yang, Ae-Rin | - |
dc.contributor.nonIdAuthor | Woo, Kyung-Hwa | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | NONCANONICAL AMINO-ACIDS | - |
dc.subject.keywordPlus | TRANSFER-RNA SYNTHETASE | - |
dc.subject.keywordPlus | GENETIC-CODE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | CONFORMATIONAL STATES | - |
dc.subject.keywordPlus | RIBOSOME | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | FRET | - |
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