A particle trapping chip using the wide and uniform slit formed by a deformable membrane with air bubble plugs

Abstract

We present a high-efficient particle trapping chip, where a wide and uniform slit is formed by a deformable membrane barrier with air bubble plugs. The previous particle trapping methods based on membrane barriers resulted in low trapping efficiency due to the non-uniform slit gap between the membrane and the substrate, especially at the side walls of rectangular channel. In the present method, the air bubble plugs remained in the extended microchannel during sample filling process, block the particle passage at the both side ends of the membrane, thus all particles flow through the uniform slit gap. Therefore, high-efficient particle trapping without particle loss can be achieved. The present particle trapping chip was composed of three layers: pneumatic (top), membrane and channel (bottom) layers. The membrane was deformed by the pneumatic pressure applied from the top layer. In the experimental study using 10.3 mu m-diameter polystyrene beads, the membrane barrier with the air bubble plugs successfully trapped the injected beads with the trapping efficiency of 100% at the flow rate of 10 mu l/min, while the barrier without the air bubble plugs showed low efficiency of 20%. At the increased flow rate of 20 mu l/min, beads were still trapped with trapping efficiency over 98% in the present device. By using a mixture of 5.7 and 10.3 mu m-diameter beads, we also verified the present method was capable to trap and release the beads selectively according to their size with the release efficiency of 95.1%. The present simple and effective particle trapping device is applicable for the high-efficient bioparticle isolation and recovery in the micro total analysis system. (C) 2013 Elsevier B. V. All rights reserved.