Development of apoptosis-resistant CHO cell line expressing PyLT for the enhancement of transient antibody production

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dc.contributor.authorKim, Won-Heeko
dc.contributor.authorKim, Min-Sooko
dc.contributor.authorKim, Yeon-Guko
dc.contributor.authorLee, Gyun-Minko
dc.date.accessioned2013-06-07T08:17:38Z-
dc.date.available2013-06-07T08:17:38Z-
dc.date.created2013-02-25-
dc.date.created2013-02-25-
dc.date.issued2012-12-
dc.identifier.citationPROCESS BIOCHEMISTRY, v.47, no.12, pp.2557 - 2561-
dc.identifier.issn1359-5113-
dc.identifier.urihttp://hdl.handle.net/10203/173887-
dc.description.abstractIn an effort to improve transient gene expression (TGE) in Chinese hamster ovary (CHO) cells, the combinatorial engineering of polyoma virus large T-antigen (PyLT) and Bcl-x(L) in CHO DG 44 cells was performed. The developed cell line (CHOP-off-Bcl-x(L)), which constitutively expresses PyLT and inducibly expresses Bcl-x(L), is capable of episomal replication with the use of the DNA expression vector encoding PyOri, EBNA-1, and OriP (pWP-Ang-EBNA/OriP-PyOri) and it is apoptosis-resistant. When the recombinant antibody was transiently expressed, this combinatorial engineering in the CHO cells resulted in a more than twofold increase in the product titer in various culture conditions such as batch, fed-batch, and cultures with sodium butyrate additions. Taken together, the data obtained here demonstrate that the use of the CHOP-off-Bcl-xL cell line can enhance the TGE significantly, which facilitates early stage product development in the pharmaceutical industry. (C) 2012 Elsevier Ltd. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCI LTD-
dc.subjectHAMSTER OVARY CELLS-
dc.subjectRECOMBINANT PROTEIN-PRODUCTION-
dc.subjectMAMMALIAN-CELLS-
dc.subjectGENE-EXPRESSION-
dc.subjectCULTURE-
dc.subjectTRANSFECTION-
dc.subjectAUTOPHAGY-
dc.subjectPERSPECTIVES-
dc.subjectBCL-X(L)-
dc.subjectSYSTEM-
dc.titleDevelopment of apoptosis-resistant CHO cell line expressing PyLT for the enhancement of transient antibody production-
dc.typeArticle-
dc.identifier.wosid000313851700116-
dc.identifier.scopusid2-s2.0-84870849449-
dc.type.rimsART-
dc.citation.volume47-
dc.citation.issue12-
dc.citation.beginningpage2557-
dc.citation.endingpage2561-
dc.citation.publicationnamePROCESS BIOCHEMISTRY-
dc.identifier.doi10.1016/j.procbio.2012.08.005-
dc.contributor.localauthorLee, Gyun-Min-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorChinese hamster ovary (CHO) cells-
dc.subject.keywordAuthorTransient gene expression (TGE)-
dc.subject.keywordAuthorPolyomavirus large-T antigen (PyLT)/PyOri system-
dc.subject.keywordAuthorEpstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1)/OriP-
dc.subject.keywordAuthorBcl-x(L)-
dc.subject.keywordPlusHAMSTER OVARY CELLS-
dc.subject.keywordPlusRECOMBINANT PROTEIN-PRODUCTION-
dc.subject.keywordPlusMAMMALIAN-CELLS-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusCULTURE-
dc.subject.keywordPlusTRANSFECTION-
dc.subject.keywordPlusAUTOPHAGY-
dc.subject.keywordPlusPERSPECTIVES-
dc.subject.keywordPlusBCL-X(L)-
dc.subject.keywordPlusSYSTEM-
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