Analysis of the Genome-Scale Metabolic Model of Pichia pastoris

Abstract

Here we present the analysis of the genome-scale metabolic model of the yeast Pichia pastoris, an attractive host for heterologous protein production and capable of producing proteins of therapeutic value. Using the genome-scale metabolic model, characteristics and capacity towards the production of two proteins, human serum albumin (HSA) and human superoxide dismutase (hSOD), were analyzed and metabolic engineering targets were suggested to improve their production. Using genome-scale metabolic model, potential phenotypes can be screen in a high-throughput manner, accelerating the development of strategies for engineering improved strains.