Comparative Analysis of Envelope Proteomes in Escherichia coli B and K-12 Strains

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 420
  • Download : 0
We analyzed the envelope proteomes of E. coli BL21(DE3) and MG1655. A total of 165 protein spots, including 62 non-redundant proteins, were unambiguously identified by 2DE and LC-MS/MS. Of these, 43 proteins were conserved between the two strains, while 4 and 16 strain-specific proteins were identified only in E. coli BL21(DE3) and MG1655, respectively. Additionally, 24 proteins showed more than 2-fold differences in intensities between the B and K-12 strains. The reference envelope proteome maps showed that E. coli envelope mainly contained channel proteins and lipoproteins. Interesting proteomic observations between the two strains will be discussed. These differences may influence the permeability and integrity of the cell envelope, showing that E. coli B may be more susceptible than K-12 to certain stress conditions. [This work was supported by the Basic Science Research Program (2010-0008826) and Converging Research Center Program (2009-0093652) through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology]
Publisher
한국화학공학회
Issue Date
2012-04
Language
KOR
Citation

2012 한국화학공학회 춘계 학술대회

URI
http://hdl.handle.net/10203/171767
Appears in Collection
CBE-Conference Papers(학술회의논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0