Transcriptome analysis of succinate-resistant mutant in Mannheimia succiniproducens

Abstract

A genetically engineered M. succiniciproducens strain for succinate production was adapted to improve succinate-resistance. Immediate and delayed responses of the parent strain and the adapted mutant to succinate were analyzed in transcriptome level for further consideration of succinate tolerance of M. succiniciproducens. Gene expression levels of the adapted strain were compared with a parent strain and the expression patterns after acid shock were classified refer to function of genes. Several genetic modifications to M. succiniciproducenswere also tried to enhancing succinate-resistance based on the transcriptome analysis.