DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gee, Heon Yung | ko |
dc.contributor.author | Kim, Young Woong | ko |
dc.contributor.author | Jo, Min Jae | ko |
dc.contributor.author | Namkung, Wan | ko |
dc.contributor.author | Kim, Joo Young | ko |
dc.contributor.author | Park, Hyun Woo | ko |
dc.contributor.author | Kim, Kyung Sik | ko |
dc.contributor.author | Kim, Hoguen | ko |
dc.contributor.author | Baba, Akemichi | ko |
dc.contributor.author | Yang, Jinhee | ko |
dc.contributor.author | Kim, Eunjoon | ko |
dc.contributor.author | Kim, Kyung Hwan | ko |
dc.contributor.author | Lee, Min Goo | ko |
dc.date.accessioned | 2010-03-10T07:41:03Z | - |
dc.date.available | 2010-03-10T07:41:03Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-08 | - |
dc.identifier.citation | GASTROENTEROLOGY, v.137, no.2, pp.607 - 617 | - |
dc.identifier.issn | 0016-5085 | - |
dc.identifier.uri | http://hdl.handle.net/10203/17079 | - |
dc.description.abstract | BACKGROUND & AIMS: Vasoactive intestinal polypeptide (VIP) is a principal regulator of fluid and electrolyte secretion in the gastrointestinal system. The VIP type-1 receptor (VPAC(1)), a class II G-protein-coupled receptor, contains a putative C-terminal PDZ-binding motif. A yeast 2-hybrid screen indicated that the C-terminus of VPAC(1) bound to the PDZ domain of synaptic scaffolding molecule (S-SCAM, also known as membrane-associated guanylate kinase inverted-2 [MAGI-2]). We analyzed the association between S-SCAM and VPAC(1). METHODS: The biochemical properties and physiologic significance of the interaction between VPAC(1) and S-SCAM were examined in heterologous expression systems, T84 colonic epithelial cells, and human pancreas and colon tissues using an integrated molecular and physiologic approach. RESULTS: The physical interaction between VPAC(1) and S-SCAM was confirmed by immunoprecipitation in HEK 293 mammalian cells and human pancreatic and colonic tissues. Immunocytochemical analysis indicated that S-SCAM recruited VPAC(1) to the junctional area near the apical end of the lateral membrane in T84 cells. Several lines of evidence revealed that S-SCAM inhibits VPAC(1) activation. Overexpression of S-SCAM inhibited VPAC(1)-mediated cAMP production and agonist-induced VPAC(1) internalization in HEK 293 and HeLa cells. In addition, S-SCAM decreased the VPAC(1)-mediated current through the cystic fibrosis transmembrane conductance regulator in Xenopus oocytes, especially at low concentrations of VIP. Importantly, loss of S-SCAM increased VIP-induced short-circuit currents in T84 monolayers, which endogenously express VPAC(1) and S-SCAM. CONCLUSIONS: S-SCAM/MAGI-2 interacts with and regulates VPAC(1) intracellular localization in epithelial cells and inhibits VPAC(1) agonist-induced activation and internalization. | - |
dc.description.sponsorship | Supported by grants KRF-2005-015-E00067 from the Korea Research Foundation and R11-2007-040-01001-0 and R01-2007000-20710-0 from the Korea Science and Engineering Foundation, Ministry of Education, Science and Technology, Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | W B SAUNDERS CO-ELSEVIER INC | - |
dc.subject | TRANSMEMBRANE CONDUCTANCE REGULATOR | - |
dc.subject | PROTEIN-COUPLED RECEPTORS | - |
dc.subject | CYCLASE-ACTIVATING POLYPEPTIDE | - |
dc.subject | CYSTIC-FIBROSIS | - |
dc.subject | GUANYLATE KINASES | - |
dc.subject | VPAC RECEPTORS | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | PEPTIDE | - |
dc.subject | FAMILY | - |
dc.subject | INTERNALIZATION | - |
dc.title | Synaptic Scaffolding Molecule Binds to and Regulates Vasoactive Intestinal Polypeptide Type-1 Receptor in Epithelial Cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000268551000036 | - |
dc.identifier.scopusid | 2-s2.0-67650951122 | - |
dc.type.rims | ART | - |
dc.citation.volume | 137 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 607 | - |
dc.citation.endingpage | 617 | - |
dc.citation.publicationname | GASTROENTEROLOGY | - |
dc.identifier.doi | 10.1053/j.gastro.2009.01.065 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Eunjoon | - |
dc.contributor.nonIdAuthor | Gee, Heon Yung | - |
dc.contributor.nonIdAuthor | Kim, Young Woong | - |
dc.contributor.nonIdAuthor | Jo, Min Jae | - |
dc.contributor.nonIdAuthor | Namkung, Wan | - |
dc.contributor.nonIdAuthor | Kim, Joo Young | - |
dc.contributor.nonIdAuthor | Park, Hyun Woo | - |
dc.contributor.nonIdAuthor | Kim, Kyung Sik | - |
dc.contributor.nonIdAuthor | Kim, Hoguen | - |
dc.contributor.nonIdAuthor | Baba, Akemichi | - |
dc.contributor.nonIdAuthor | Kim, Kyung Hwan | - |
dc.contributor.nonIdAuthor | Lee, Min Goo | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | TRANSMEMBRANE CONDUCTANCE REGULATOR | - |
dc.subject.keywordPlus | PROTEIN-COUPLED RECEPTORS | - |
dc.subject.keywordPlus | CYCLASE-ACTIVATING POLYPEPTIDE | - |
dc.subject.keywordPlus | CYSTIC-FIBROSIS | - |
dc.subject.keywordPlus | GUANYLATE KINASES | - |
dc.subject.keywordPlus | VPAC RECEPTORS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | FAMILY | - |
dc.subject.keywordPlus | INTERNALIZATION | - |
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