Measurement of enzyme activities related in succinic acid production of Mannheimia Succiniciproducens MBEL55E

Abstract

Mannheimia succiniciproducens MBEL55E, a capnophilic rumen bacterium, produces succinic acid as a major fermentation product during anaerobic cultivation. M. succiniciproducens has been known to use anaplerotic pathway to produce succinic acid. In the starting point of the anaplerotic pathway, two Enzymes, phosphoenolpyruvate carboxylase(Ppc) and phosphoenolpyruvate kinase(PckA), convert phosphoenolpyruvate to oxaloacetate. We analysed activation of both enzymes to elucidate the roles in the production of succinic acid. This study shows that PckA is 50 times stronger than Ppc in M. succiniciproducens, suggesting that PckA is a very important enzyme for producing succinic acid.