Role of the sequence of the rne-dependent site in 3 processing of M1 RNA, the catalytic component of Escherichia coli RNase P

Abstract

The 3' processing of MI RNA, the catalytic component of Escherichia coli RNase P, occurs by two pathways involving multiple steps. The precursor of MI RNA has an rne-dependent site downstream of the processing site, whose sequence variation affects the processing efficiency. In this study, we showed that the sequence itself of the rue-dependent site possessed the ability to determine the processing pathways and that it also affected the cleavage specificity with the generation of the processing products at one nucleotide upstream or downstream of the normal cleavage sites. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.