Succinic acid has recently been drawing much interest as a raw material for biodegradable polymer. This study deals with the development of purification and separation processes required to produce the highly purified succinic acid from the fermentation broth produced by recombinant microorganism, Mannheimia succiniciproducens. The developed process consists of the pretreatment step of vacuum distillation and the crystallization step for the highly purified succinic acid production. Fermentation broth, separated from cells in the fermentation broth by the centrifugation, is applied to the pretreatment processes which is the vacuum distillation for concentration and removal of the volatile contaminated organic acid. The crystallization of highly purified succinic acid from the pretreatment sample solution is conducted at adjusting the pH.