DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Kyung Chul | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Chung, Hyun Jung | - |
dc.contributor.author | Joe, Cheol O | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.contributor.author | Park, Tae Gwan | - |
dc.date.accessioned | 2009-12-08T02:47:32Z | - |
dc.date.available | 2009-12-08T02:47:32Z | - |
dc.date.issued | 2005-11-02 | - |
dc.identifier.citation | Journal of Controlled Release, Vol.108, No.1, pp.121-131 | en |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/14367 | - |
dc.description.abstract | Recombinant reversed caspase-3 (rev-caspase-3) is a pro-apoptotic gene capable of intracellular autocatalytic processing, which leads to programmed cell death. Folate receptor-specific intracellular delivery of the rev-caspase-3 gene into KB cells over-expressing folate receptors was explored by employing the folate–poly(ethylene glycol)–polyethylenimine (FOL–PEG–PEI) conjugate as a nonviral polymeric carrier. Using luciferase as a reporter gene, the conditions for formulation of DNA/polymer polyplexes were pre-optimized to attain the highest folate receptor-mediated gene transfection efficiency. FOL–PEG–PEI conjugate complexed with rev-caspase-3 plasmid in an optimized condition gave rise to a great increase in expression and activation of exogenous rev-caspase-3 in KB cells when pretreated with doxorubicin. The synthesized conjugate exhibited higher transfection efficiency than other commercially available transfection agents due to a unique mechanism of folate-receptor mediated endocytic gene transfer. The transfected cells showed a significant extent of apoptosis by rev-caspase-3. This study suggests the potential of using folate-receptor-mediated delivery of rev-caspase-3 gene for inducing tumor cell death in a target-specific manner. | en |
dc.description.sponsorship | Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112, USA | en |
dc.language.iso | en_US | en |
dc.publisher | Elsevier | en |
dc.subject | PEI | en |
dc.subject | folate | en |
dc.subject | Receptor-mediated endocytosis | en |
dc.subject | Rev-caspase-3 | en |
dc.subject | Apoptosis | en |
dc.title | Folate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosis | en |
dc.type | Article | en |
dc.identifier.doi | 10.1016/j.jconrel.2005.07.015 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.