Folate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosis

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dc.contributor.authorCho, Kyung Chul-
dc.contributor.authorJeong, Ji Hoon-
dc.contributor.authorChung, Hyun Jung-
dc.contributor.authorJoe, Cheol O-
dc.contributor.authorKim, Sung Wan-
dc.contributor.authorPark, Tae Gwan-
dc.date.accessioned2009-12-08T02:47:32Z-
dc.date.available2009-12-08T02:47:32Z-
dc.date.issued2005-11-02-
dc.identifier.citationJournal of Controlled Release, Vol.108, No.1, pp.121-131en
dc.identifier.issn0168-3659-
dc.identifier.urihttp://hdl.handle.net/10203/14367-
dc.description.abstractRecombinant reversed caspase-3 (rev-caspase-3) is a pro-apoptotic gene capable of intracellular autocatalytic processing, which leads to programmed cell death. Folate receptor-specific intracellular delivery of the rev-caspase-3 gene into KB cells over-expressing folate receptors was explored by employing the folate–poly(ethylene glycol)–polyethylenimine (FOL–PEG–PEI) conjugate as a nonviral polymeric carrier. Using luciferase as a reporter gene, the conditions for formulation of DNA/polymer polyplexes were pre-optimized to attain the highest folate receptor-mediated gene transfection efficiency. FOL–PEG–PEI conjugate complexed with rev-caspase-3 plasmid in an optimized condition gave rise to a great increase in expression and activation of exogenous rev-caspase-3 in KB cells when pretreated with doxorubicin. The synthesized conjugate exhibited higher transfection efficiency than other commercially available transfection agents due to a unique mechanism of folate-receptor mediated endocytic gene transfer. The transfected cells showed a significant extent of apoptosis by rev-caspase-3. This study suggests the potential of using folate-receptor-mediated delivery of rev-caspase-3 gene for inducing tumor cell death in a target-specific manner.en
dc.description.sponsorshipDepartment of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, South Korea Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112, USAen
dc.language.isoen_USen
dc.publisherElsevieren
dc.subjectPEIen
dc.subjectfolateen
dc.subjectReceptor-mediated endocytosisen
dc.subjectRev-caspase-3en
dc.subjectApoptosisen
dc.titleFolate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosisen
dc.typeArticleen
dc.identifier.doi10.1016/j.jconrel.2005.07.015-

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