Functionalization of a poly(amidoamine) dendrimer with ferrocenyls and its application to the construction of a reagentless enzyme electrode

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Poly(amidoamine) dendrimers having various degrees of modification with the redox-active ferrocenyls were prepared by controlling the molar ratio of ferrocenecarboxaldehyde to amine groups of dendrimers, By alternate layer-by layer depositions of partial ferrocenyl-tethered dendrimers (Fc-D) with periodate-oxidized glucose oxidase (GOx) on a Au surface, an electrochemically and enzymatically active multilayered assembly of enzyme was constructed. The resulting GOx/Fc-D multilayer-associated electrodes were electrochemically analyzed, and the surface concentration of ferrocenyl groups, active enzyme coverage, and sensitivity were estimated. A 32% dendrimer modification level of surface amines to ferrocenyls was found to be an optimum in terms of enzyme-dendrimer network formation, electrochemical interconnectivity of ferrocenyls, and electrode sensitivity. With the prepared Fc(32%)-tethered dendrimers, mono- and multilayered GOx/Fc-D electrodes were constructed, and their electrochemical and catalytic properties were characterized. The bioelectrocatalytic signals from the multilayered GOx/Fc-D electrodes were shown to be directly correlated to the number of deposited bilayers. From this result, it seems that the electrode sensitivity is directly controllable, and the multilayer-forming strategy with partial ferrocenyl-tethered dendrimers is useful for the construction of reagentless biosensors.
Publisher
AMER CHEMICAL SOC
Issue Date
2000
Language
English
Article Type
Article
Keywords

STARBURST DENDRIMERS; GOLD; FILMS; ASSEMBLIES; ADSORPTION; MONOLAYERS; BEHAVIOR; PRODUCT; SIZE

Citation

ANALYTICAL CHEMISTRY, v.72, no.18, pp.4420 - 4427

ISSN
0003-2700
DOI
10.1021/ac0003044
URI
http://hdl.handle.net/10203/14248
Appears in Collection
BS-Journal Papers(저널논문)
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