Protein kinase assay on peptide-conjugated gold nanoparticles

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dc.contributor.authorKim, Young-Pilko
dc.contributor.authorOh, Young-Heeko
dc.contributor.authorKim, Hak-Sungko
dc.date.accessioned2009-12-07T01:05:32Z-
dc.date.available2009-12-07T01:05:32Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2008-02-
dc.identifier.citationBIOSENSORS BIOELECTRONICS, v.23, no.7, pp.980 - 986-
dc.identifier.issn0956-5663-
dc.identifier.urihttp://hdl.handle.net/10203/14244-
dc.description.abstractWe demonstrate that protein kinase can be assayed with high sensitivity on peptide-conjugated gold nanoparticles (AuNPs). Phosphorylation of peptides on the AuNP-monolayers was detected by using an anti-phosphotyrosine antibody (alpha-pY) and Cy3-labeled secondary antibody (Cy3-alpha-mIgG) as a probing molecule. When compared to conventional self-assembled monolayers; (SAMs), spherical and three-dimensional geometry of AuNPs led to high surface density of peptide substrate and easy accessibility to enzyme, and consequently the resulting AuNP monolayers gave rise to improved detection sensitivity. Blocking of peptide-conjugated AuNPs with a poly(ethylene glycol) (PEG) also contributed to a higher signal-to-background ratio in kinase and its inhibition assays. The use of AuNPs as the platform surface will enable highly sensitive detection of protein kinases in a high-throughput manner. (C) 2007 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherELSEVIER ADVANCED TECHNOLOGY-
dc.subjectLIGAND INTERACTIONS-
dc.subjectMONOLAYERS-
dc.subjectSURFACES-
dc.subjectARRAYS-
dc.subjectMICROARRAYS-
dc.subjectANTIGEN-
dc.titleProtein kinase assay on peptide-conjugated gold nanoparticles-
dc.typeArticle-
dc.identifier.wosid000253620900009-
dc.identifier.scopusid2-s2.0-38749095231-
dc.type.rimsART-
dc.citation.volume23-
dc.citation.issue7-
dc.citation.beginningpage980-
dc.citation.endingpage986-
dc.citation.publicationnameBIOSENSORS BIOELECTRONICS-
dc.identifier.doi10.1016/j.bios.2007.10.001-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorKim, Hak-Sung-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorprotein kinase-
dc.subject.keywordAuthorphosphorylation-
dc.subject.keywordAuthorgold nanoparticles-
dc.subject.keywordAuthorpeptide-
dc.subject.keywordAuthorfluorescent assay-
dc.subject.keywordPlusLIGAND INTERACTIONS-
dc.subject.keywordPlusMONOLAYERS-
dc.subject.keywordPlusSURFACES-
dc.subject.keywordPlusARRAYS-
dc.subject.keywordPlusMICROARRAYS-
dc.subject.keywordPlusANTIGEN-
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