DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, KC | ko |
dc.contributor.author | Kim, SH | ko |
dc.contributor.author | Jeong, JH | ko |
dc.contributor.author | Park, Tae Gwan | ko |
dc.date.accessioned | 2009-11-25T03:00:02Z | - |
dc.date.available | 2009-11-25T03:00:02Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-06 | - |
dc.identifier.citation | MACROMOLECULAR BIOSCIENCE, v.5, no.6, pp.512 - 519 | - |
dc.identifier.issn | 1616-5187 | - |
dc.identifier.uri | http://hdl.handle.net/10203/13311 | - |
dc.description.abstract | For efficient receptor-mediated gene transfection, a new and simple formulation method based on using PEI and FOLPEGPLL conjugate was presented. Luciferase plasmid DNA and PEI were complexed to form slightly positive-charged nanoparticles, onto which FOL-PEG-PLL conjugate was surface coated. With increasing the coating amount of FOL-PEG-PLL conjugate, the FOL-PEG-PLL/ PEI/DNA complexes exhibited increased surface zeta-potential values with concomitantly increased diameters, indicating that the PLL part was physically anchored on the surface of preformed PEI/DNA complexes with FOL moieties being exposed on the outside. The formulated complexes exhibited a considerably higher transfection efficiency against FOL receptor over-expressing KB cells than FOL receptor deficient A549 cells. This was caused by an enhanced cellular uptake of the resultant complexes via a receptor-mediated endocytosis process. The formulated complexes showed a higher gene expression level, even in the presence of serum, than the PEI/DNA or Lipofectamine/DNA complexes. This was attributed to the PEG chains present on the surface of complexes that could work as a protective shield layer against aggregation caused by non-specific protein adsorption. The FOL-PEG-PLL/PEI/DNA complexes also demonstrated better cell viability than the PEI/DNA complexes. | - |
dc.description.sponsorship | the grant from the Ministry of Science and Technology, Republic of Korea and the Korean Science and Engineering Foundation (KOSEF). | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | EPIDERMAL GROWTH-FACTOR | - |
dc.subject | IN-VIVO | - |
dc.subject | TRANSFECTION EFFICIENCY | - |
dc.subject | CATIONIC POLYMERS | - |
dc.subject | BLOCK-COPOLYMER | - |
dc.subject | NUCLEIC-ACIDS | - |
dc.subject | T-LYMPHOCYTES | - |
dc.subject | PLASMID DNA | - |
dc.subject | POLYETHYLENIMINE | - |
dc.subject | CELLS | - |
dc.title | Folate receptor-mediated gene delivery using folate-poly(ethylene glycol)-poly (L-lysine) conjugate | - |
dc.type | Article | - |
dc.identifier.wosid | 000230273200005 | - |
dc.identifier.scopusid | 2-s2.0-21644487837 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 512 | - |
dc.citation.endingpage | 519 | - |
dc.citation.publicationname | MACROMOLECULAR BIOSCIENCE | - |
dc.identifier.doi | 10.1002/mabi.200500018 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Tae Gwan | - |
dc.contributor.nonIdAuthor | Cho, KC | - |
dc.contributor.nonIdAuthor | Kim, SH | - |
dc.contributor.nonIdAuthor | Jeong, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | folate | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | poly(ethylenimine) (PEI) | - |
dc.subject.keywordAuthor | receptor-mediated endocytosis | - |
dc.subject.keywordPlus | EPIDERMAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | CATIONIC POLYMERS | - |
dc.subject.keywordPlus | BLOCK-COPOLYMER | - |
dc.subject.keywordPlus | NUCLEIC-ACIDS | - |
dc.subject.keywordPlus | T-LYMPHOCYTES | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | CELLS | - |
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