DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Haeshin | ko |
dc.contributor.author | Park, TG | ko |
dc.date.accessioned | 2009-11-25T02:37:05Z | - |
dc.date.available | 2009-11-25T02:37:05Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2003-01 | - |
dc.identifier.citation | JOURNAL OF PHARMACEUTICAL SCIENCES, v.92, no.11, pp.97 - 103 | - |
dc.identifier.issn | 0022-3549 | - |
dc.identifier.uri | http://hdl.handle.net/10203/13303 | - |
dc.description.abstract | The identification of PEGylation sites is essential in the characterization of PEGylated therapeutic proteins. This report describes a simple and novel method of finding poly(ethylene glycol) (PEG) conjugation sites in PEGylated proteins by using a hetero-fanctional biotin-PEG-N-hydroxyl succinimide derivative. PEGylated lysozyme species having a biotin moiety at each PEG chain end were separated and digested by trypsin. Among the digested lysozyme fragments, biotin-terminated PEGylated peptide fragments were purified by a monomeric avidin immobilized column. Their mass was analyzed by matrix-assisted laser desorption ionization time of flight mass spectrometry, directly indicating that PEG was conjugated to lysine 33, 97, 116 residues. Reversed-phase high-pressure liquid chromatography results for the PEGylated peptide fragments exhibited that PEGylation occurred preferentially at lysine 33> lysine 97> lysine 116. (C) 2002 Wiley-Liss, Inc. | - |
dc.description.sponsorship | the Center for Advanced Functional Polymers, KAIST, and the Ministry of Commerce, Industry, and Energy (A00-961-5411-02-3-3), Republic of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | JOHN WILEY & SONS INC | - |
dc.subject | POLYETHYLENE-GLYCOL | - |
dc.subject | SUPEROXIDE-DISMUTASE | - |
dc.subject | COVALENT ATTACHMENT | - |
dc.subject | POSITIONAL ISOMERS | - |
dc.subject | IMMUNOLOGICAL PROPERTIES | - |
dc.subject | POLY(ETHYLENE GLYCOL) | - |
dc.subject | CHEMICAL MODIFICATION | - |
dc.subject | STABILITY | - |
dc.subject | LYSOZYME | - |
dc.subject | PEPTIDE | - |
dc.title | A novel method for identifying PEGylation sites of protein using biotinylated PEG derivatives | - |
dc.type | Article | - |
dc.identifier.wosid | 000180207200014 | - |
dc.identifier.scopusid | 2-s2.0-0037223763 | - |
dc.type.rims | ART | - |
dc.citation.volume | 92 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 97 | - |
dc.citation.endingpage | 103 | - |
dc.citation.publicationname | JOURNAL OF PHARMACEUTICAL SCIENCES | - |
dc.identifier.doi | 10.1002/jps.10270 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Lee, Haeshin | - |
dc.contributor.localauthor | Park, TG | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | polyethylene glycol (PEG) | - |
dc.subject.keywordAuthor | identification of PEGylation sites | - |
dc.subject.keywordAuthor | avidin-biotin | - |
dc.subject.keywordAuthor | MALDI-TOF MS | - |
dc.subject.keywordPlus | POLYETHYLENE-GLYCOL | - |
dc.subject.keywordPlus | SUPEROXIDE-DISMUTASE | - |
dc.subject.keywordPlus | COVALENT ATTACHMENT | - |
dc.subject.keywordPlus | POSITIONAL ISOMERS | - |
dc.subject.keywordPlus | IMMUNOLOGICAL PROPERTIES | - |
dc.subject.keywordPlus | POLY(ETHYLENE GLYCOL) | - |
dc.subject.keywordPlus | CHEMICAL MODIFICATION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | LYSOZYME | - |
dc.subject.keywordPlus | PEPTIDE | - |
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