Energy transfer-based multiplexed assay of proteases by using gold nanoparticle and quantum dot conjugates on a surface

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Rapid and sensitive assay of proteases and their inhibition in a high-throughput manner is of great significance in the diagnostic and pharmaceutical fields. We developed a multiplexed assay system of proteases and their inhibition by measuring the energy transfer between quantum dots (QDs) and gold nanoparticles (AuNPs) on a glass slide. In this system, while the photoluminescence (PL) of donor QDs immobilized on a surface was quenched due to the presence of AuNPs (energy acceptor) in close proximity, the protease activity caused modulation in the efficiency of the energy transfer between the acceptor and donor, thus enabling the protease assay. In comparison to the QD-dye system, the conjugate of the QD-AuNP gave rise to higher energy transfer efficiency, resulting in quantitative assay of proteases with more sensitivity. When matrix metalloproteinase, caspase, and thrombin were tested, a multiplexed assay was successfully achieved since the AuNP could be used as a common energy acceptor in conjunction with QDs having different colors. Our system is anticipated to find applications in the diagnosis of protease-related diseases and screening of potential drugs with high sensitivity in a high-throughput way.
Publisher
AMER CHEMICAL SOC
Issue Date
2008-06
Language
English
Article Type
Article
Keywords

MATRIX METALLOPROTEINASES; PROTEOLYTIC ACTIVITY; FRET; INHIBITION; PROBES

Citation

ANALYTICAL CHEMISTRY, v.80, no.12, pp.4634 - 4641

ISSN
0003-2700
DOI
10.1021/ac702416e
URI
http://hdl.handle.net/10203/13238
Appears in Collection
BS-Journal Papers(저널논문)
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