DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sun-Hwa | ko |
dc.contributor.author | Jeong, Ji-Hoon | ko |
dc.contributor.author | Mok, Hye-Jung | ko |
dc.contributor.author | Lee, Soo-Hyeon | ko |
dc.contributor.author | Kim, Sung-Wan | ko |
dc.contributor.author | Park, Tae-Gwan | ko |
dc.date.accessioned | 2009-11-24T06:48:30Z | - |
dc.date.available | 2009-11-24T06:48:30Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS, v.23, no.1, pp.232 - 237 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | http://hdl.handle.net/10203/13223 | - |
dc.description.abstract | A polyelectrolyte complex micelle (PECM)-based delivery system for targeting folate (FOL) receptor overexpressing tumor cells is demonstrated using poly(ethylene glycol) (PEG)-conjugated oligonucleotide (ODN). The tumor targeting property was conferred to the PECM by tethering a folate moiety to the distal end of the PEG segment in an anti-sense green fluorescent protein (GFP) ODN-PEG conjugate. Nanoscale PECMs were spontaneously produced from ionic interactions between the ODN-PEG-FOL conjugate and a cationic lipid, lipofectamine (Lf). When treated with FOL receptor overexpressing cells (KB), the PCEMs caused a significant reduction in GFP expression in a dose-dependent manner. This effect was not observed in FOL receptor deficient cells (A549). The enhanced transfection of ODN-PEG-FOL/Lf PECMs to KB cells was caused by FOL receptor mediated endocytosis. The efficiency of target-specific gene suppression by ODN-PEG-FOL/Lf PECMs was maintained even in the presence of 10% fetal bovine serum in the transfection medium. | - |
dc.description.sponsorship | National Research Laboratory Grant from the Ministry of Science and Technology, Republic of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Wiley-Blackwell | - |
dc.subject | BLOCK-COPOLYMER MICELLES | - |
dc.subject | GENE DELIVERY | - |
dc.subject | ANTISENSE OLIGONUCLEOTIDES | - |
dc.subject | INTRACELLULAR DELIVERY | - |
dc.subject | GLYCOL) CONJUGATE | - |
dc.subject | CELLULAR UPTAKE | - |
dc.subject | DRUG | - |
dc.subject | DNA | - |
dc.subject | POLY(ETHYLENIMINE) | - |
dc.subject | STABILIZATION | - |
dc.title | Folate receptor targeted delivery of polyelectrolyte complex micelles prepared from ODN-PEG-Folate conjugate and cationic lipids | - |
dc.type | Article | - |
dc.identifier.wosid | 000243927600032 | - |
dc.identifier.scopusid | 2-s2.0-33847168570 | - |
dc.type.rims | ART | - |
dc.citation.volume | 23 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 232 | - |
dc.citation.endingpage | 237 | - |
dc.citation.publicationname | BIOTECHNOLOGY PROGRESS | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Tae-Gwan | - |
dc.contributor.nonIdAuthor | Kim, Sun-Hwa | - |
dc.contributor.nonIdAuthor | Jeong, Ji-Hoon | - |
dc.contributor.nonIdAuthor | Mok, Hye-Jung | - |
dc.contributor.nonIdAuthor | Kim, Sung-Wan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | BLOCK-COPOLYMER MICELLES | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | ANTISENSE OLIGONUCLEOTIDES | - |
dc.subject.keywordPlus | INTRACELLULAR DELIVERY | - |
dc.subject.keywordPlus | GLYCOL) CONJUGATE | - |
dc.subject.keywordPlus | CELLULAR UPTAKE | - |
dc.subject.keywordPlus | DRUG | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | POLY(ETHYLENIMINE) | - |
dc.subject.keywordPlus | STABILIZATION | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.