Injectable Cellular Aggregates Prepared from Biodegradable Porous Microspheres for Adipose Tissue Engineering

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Cellular aggregates were prepared using biodegradable porous microspheres for injectable reconstruction of soft tissues in vivo. Biodegradable porous microspheres with sizes of similar to 50 mu m were prepared by a porogen leaching-phase separation process in an oil-in-water single-emulsion method using poly( D, L-lactide-co-glycolide). 3T3 L1 mouse preadipocyte cells were transformed into cellular aggregates by suspension cultivation in a spinner flask using the porous microspheres as effective buoyant and cell-adhesive microcarriers. Spherically shaped aggregates were readily formed with sizes of up to 1000 mu m with a higher number of viable cells compared to the nonporous microspheres. The resultant cellular aggregates exhibited far better extent of differentiation into adipocytes than the cell aggregates prepared from nonporous microspheres or monolayer cultured cells. The cellular aggregates could also be injected into nude mice using a syringe needle for adipose tissue regeneration in vivo. Immunohistochemical studies showed that the aggregate-derived tissues with porous microspheres displayed histological characteristics similar to adipose tissues 4 weeks after injection.
Publisher
MARY ANN LIEBERT INC
Issue Date
2009-06
Language
English
Article Type
Article
Keywords

MESENCHYMAL STEM-CELLS; POLYMERIC MICROSPHERES; SUSPENSION-CULTURE; SCAFFOLDS; MICROCARRIER; GROWTH; IMPLANTATION; CULTIVATION; MATRIX; CHONDROCYTES

Citation

TISSUE ENGINEERING PART A, v.15, no.6, pp.1391 - 1400

ISSN
1076-3279
DOI
10.1089/ten.tea.2008.0344
URI
http://hdl.handle.net/10203/13129
Appears in Collection
NT-Journal Papers(저널논문)BS-Journal Papers(저널논문)
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