Galactosylated polyethylenimine-graft-poly(vinyl pyrrolidone) as ahepatocyte-targeting gene carrier

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dc.contributor.authorCook, SEko
dc.contributor.authorPark, IKko
dc.contributor.authorKim, EMko
dc.contributor.authorJeong, HJko
dc.contributor.authorPark, TGko
dc.contributor.authorChoi, YJko
dc.contributor.authorAkaike, Tko
dc.contributor.authorCho, CSko
dc.date.accessioned2009-11-23T05:26:08Z-
dc.date.available2009-11-23T05:26:08Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2005-06-
dc.identifier.citationJOURNAL OF CONTROLLED RELEASE, v.105, no.1-2, pp.151 - 163-
dc.identifier.issn0168-3659-
dc.identifier.urihttp://hdl.handle.net/10203/13113-
dc.description.abstractPolyethylenimine (PEI) has been used for the gene delivery system in vitro and in vivo since it has high transfection efficiency owing to proton buffer capacity. However, the use of PEI for gene delivery is limited due to cytotoxicity, non-specificity and unnecessary interaction with serum components. To overcome cytotoxicity and non-specificity, PEI was coupled with poly(vinyl pyrrolidone) (PVP) as the hydrophilic group to reduce cytotoxicity and lactose bearing galactose group for hepatocyte targeting. The galactosylated-PEI-graft-PVP (GPP) was complexed with DNA, and GPP/DNA complexes were characterized. GPP showed good DNA binding ability, high protection of DNA from nuclease attack. The sizes of DNA complexes show tendency to decrease with an increase of charge ratio and had a minimum value around 59 nin at the charge ratio of 40 for the GPP-1/DNA complex (PVP content: 4.1 mol%). The GPP showed low cytotoxicity. And GPP/DNA complexes were mediated by asialoglycoprotein receptors (ASGP-R)-mediated endocytosis. Also, the transfection efficiency of GPP-1/DNA complex at charge ratio of 40 in the HepG2 was higher than that of PEI/DNA one. (c) 2005 Elsevier B.V. All rights reserved.-
dc.description.sponsorshipthe fund provided by Ministry of Science and Technologyen
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherElsevier Science Bv-
dc.subjectPOLY-L-LYSINE-
dc.subjectIN-VIVO-
dc.subjectPLASMID DNA-
dc.subjectASIALOGLYCOPROTEIN RECEPTOR-
dc.subjectHEPATOMA-CELLS-
dc.subjectDELIVERY-
dc.subjectCOMPLEXES-
dc.subjectHEPATOCYTES-
dc.subjectPOLYETHYLENIMINE-
dc.subjectEXPRESSION-
dc.titleGalactosylated polyethylenimine-graft-poly(vinyl pyrrolidone) as ahepatocyte-targeting gene carrier-
dc.typeArticle-
dc.identifier.wosid000230576600014-
dc.identifier.scopusid2-s2.0-20444426861-
dc.type.rimsART-
dc.citation.volume105-
dc.citation.issue1-2-
dc.citation.beginningpage151-
dc.citation.endingpage163-
dc.citation.publicationnameJOURNAL OF CONTROLLED RELEASE-
dc.identifier.doi10.1016/j.jconrel.2005.03.011-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorPark, TG-
dc.contributor.nonIdAuthorCook, SE-
dc.contributor.nonIdAuthorPark, IK-
dc.contributor.nonIdAuthorKim, EM-
dc.contributor.nonIdAuthorJeong, HJ-
dc.contributor.nonIdAuthorChoi, YJ-
dc.contributor.nonIdAuthorAkaike, T-
dc.contributor.nonIdAuthorCho, CS-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorgene carrier-
dc.subject.keywordAuthorpolyethylenimine-
dc.subject.keywordAuthorgalactose-
dc.subject.keywordAuthorpoly (vinyl pyrrolidone)-
dc.subject.keywordAuthorasialoglycoprotein-receptor-
dc.subject.keywordPlusPOLY-L-LYSINE-
dc.subject.keywordPlusIN-VIVO-
dc.subject.keywordPlusPLASMID DNA-
dc.subject.keywordPlusASIALOGLYCOPROTEIN RECEPTOR-
dc.subject.keywordPlusHEPATOMA-CELLS-
dc.subject.keywordPlusDELIVERY-
dc.subject.keywordPlusCOMPLEXES-
dc.subject.keywordPlusHEPATOCYTES-
dc.subject.keywordPlusPOLYETHYLENIMINE-
dc.subject.keywordPlusEXPRESSION-
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