DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SH | ko |
dc.contributor.author | Jeong, JH | ko |
dc.contributor.author | Joe, Cheol O | ko |
dc.contributor.author | Park, TG | ko |
dc.date.accessioned | 2009-11-23T05:19:09Z | - |
dc.date.available | 2009-11-23T05:19:09Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-04 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v.103, no.3, pp.625 - 634 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/13111 | - |
dc.description.abstract | To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins or other bioactive macromolecules into a specific cell, a di-block copolymer conjugate, poly(L-lysine)-polyethylene glycol)-folate (PLL-PEG-FOL), was synthesized. The PLL-PEG-FOL conjugate was physically complexed with fluorescein isothiocyanate conjugated bovine serum albumin (FITC-BSA) in an aqueous phase by ionic interactions. Cellular uptake of PLL-PEG-FOL/FITC-BSA complexes was greatly enhanced against a folate receptor over-expressing cell line (KB cells) compared to a folate receptor deficient cell line (A549 cells). The presence of an excess amount of free folate (1 mM) in the medium inhibited the intracellular delivery of PLL-PEG-FOL/FITC-BSA complexes. This suggests that the enhanced cellular uptake of FITC-BSA by KB cells in a specific manner was attributed to folate receptor-mediated endocytosis of the complexes having folate moieties on the surface. The PLL-PEG-FOL di-block copolymer could be potentially applied for intracellular delivery of a wide range of other biological active agents that have negative charges on the surface. (c) 2005 Elsevier B.V All rights reserved. | - |
dc.description.sponsorship | National Cancer Center (02-3-150) and the Ministry of Science and Technology (M10214000117- 02b1500-02110), Republic of Korea. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Elsevier Science Bv | - |
dc.subject | POLYION COMPLEX MICELLES | - |
dc.subject | ENTRAPPING ENZYME MOLECULES | - |
dc.subject | DRUG-DELIVERY | - |
dc.subject | BLOCK-COPOLYMER | - |
dc.subject | ANTISENSE OLIGONUCLEOTIDE | - |
dc.subject | GENE DELIVERY | - |
dc.subject | TARGETED DRUG | - |
dc.subject | CELLS | - |
dc.subject | PEPTIDE | - |
dc.subject | SYSTEM | - |
dc.title | Folate receptor mediated intracellular protein delivery using PLL-PEG-FOL conjugate | - |
dc.type | Article | - |
dc.identifier.wosid | 000228715500008 | - |
dc.identifier.scopusid | 2-s2.0-16344382196 | - |
dc.type.rims | ART | - |
dc.citation.volume | 103 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 625 | - |
dc.citation.endingpage | 634 | - |
dc.citation.publicationname | JOURNAL OF CONTROLLED RELEASE | - |
dc.identifier.doi | 10.1016/j.jconrel.2004.01.006 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Joe, Cheol O | - |
dc.contributor.localauthor | Park, TG | - |
dc.contributor.nonIdAuthor | Kim, SH | - |
dc.contributor.nonIdAuthor | Jeong, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | folate | - |
dc.subject.keywordAuthor | intracellular protein delivery | - |
dc.subject.keywordAuthor | targeting | - |
dc.subject.keywordAuthor | PLL-PEG-FOL conjugate | - |
dc.subject.keywordPlus | POLYION COMPLEX MICELLES | - |
dc.subject.keywordPlus | ENTRAPPING ENZYME MOLECULES | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | BLOCK-COPOLYMER | - |
dc.subject.keywordPlus | ANTISENSE OLIGONUCLEOTIDE | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | TARGETED DRUG | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | SYSTEM | - |
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