DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Tae Hee | ko |
dc.contributor.author | Nah, Jae Woon | ko |
dc.contributor.author | Cho, Myung-Haing | ko |
dc.contributor.author | Park, Tae Gwan | ko |
dc.contributor.author | Cho, Chong Su | ko |
dc.date.accessioned | 2009-11-23T01:56:37Z | - |
dc.date.available | 2009-11-23T01:56:37Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.6, no.9-10, pp.2796 - 2803 | - |
dc.identifier.issn | 1533-4880 | - |
dc.identifier.uri | http://hdl.handle.net/10203/13082 | - |
dc.description.abstract | Dendritic cells (DCs) are professional antigen presenting cells that induce, sustain, and regulate immune responses. Gene modification of DCs is of particular interest for immunotherapy of diseases where the immunes system has failed or is abnormally regulated, such as in cancer or autoimmune disease. Gene transfer using non-viral vectors is a promising approach for the safe delivery of therapeutic DNA. Among various non-viral vectors, chitosan is considered to be a good candidate for gene delivery system, however, lack of cell specificity and low transfection of chitosan need to be overcome prior to clinical use. In this study, mannosylated chitosan (MC) was prepared to induce the receptor-mediated endocytosis and targeting into antigen presenting cells (APCs), especially DCs having mannose receptors. MC showed great ability to form complexes with DNA and showed suitable physicochemical properties for gene delivery system. It had low cytotoxicity and exhibited much enhanced gene transfer efficiency on the macrophage cell line than chitosan itself. Also, MC/DNA complex was more efficient for transferring IL-12 gene into DCs rather than water-soluble chitosan (WSC)/DNA one, which resulted in better induction of INF-gamma from DCs. Therefore, MC is a promising gene delivery system for repeated administration to maintain sustained gene expression, thereby opening the possibility for immunotherapy. | - |
dc.description.sponsorship | Ministry of Science and Technology in Korea. National Instrumentation Center for Environmental Management (NICEM) for giving opportunity of measurement of TEM. | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Amer Scientific Publishers | - |
dc.subject | DENDRITIC CELLS | - |
dc.subject | MANNOSE RECEPTOR | - |
dc.subject | IMMUNE-RESPONSES | - |
dc.subject | DNA | - |
dc.subject | TRANSFECTION | - |
dc.subject | SYSTEM | - |
dc.subject | MACROPHAGES | - |
dc.subject | TOLERANCE | - |
dc.subject | CYTOKINES | - |
dc.subject | VACCINES | - |
dc.title | Receptor-mediated gene delivery into antigen presenting cells using mannosylated chitosan/DNA nanoparticles | - |
dc.type | Article | - |
dc.identifier.wosid | 000240865900018 | - |
dc.identifier.scopusid | 2-s2.0-33750074757 | - |
dc.type.rims | ART | - |
dc.citation.volume | 6 | - |
dc.citation.issue | 9-10 | - |
dc.citation.beginningpage | 2796 | - |
dc.citation.endingpage | 2803 | - |
dc.citation.publicationname | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Tae Gwan | - |
dc.contributor.nonIdAuthor | Kim, Tae Hee | - |
dc.contributor.nonIdAuthor | Nah, Jae Woon | - |
dc.contributor.nonIdAuthor | Cho, Myung-Haing | - |
dc.contributor.nonIdAuthor | Cho, Chong Su | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | receptor-mediated gene delivery | - |
dc.subject.keywordAuthor | chitosan | - |
dc.subject.keywordAuthor | mannose | - |
dc.subject.keywordAuthor | antigen presenting cells | - |
dc.subject.keywordAuthor | nanoparticle | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | MANNOSE RECEPTOR | - |
dc.subject.keywordPlus | IMMUNE-RESPONSES | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | TOLERANCE | - |
dc.subject.keywordPlus | CYTOKINES | - |
dc.subject.keywordPlus | VACCINES | - |
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