DC Field | Value | Language |
---|---|---|
dc.contributor.author | Byoung Sam Ko | ko |
dc.contributor.author | Jinmi Kim | ko |
dc.contributor.author | Kim, Jung Hoe | ko |
dc.date.accessioned | 2009-11-17T02:46:04Z | - |
dc.date.available | 2009-11-17T02:46:04Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2006-06 | - |
dc.identifier.citation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.72, pp.4207 - 4213 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.uri | http://hdl.handle.net/10203/12712 | - |
dc.description.abstract | Xylitol dehydrogenase (XDH) is one of the key enzymes in D-Xylose metabolism, catalyzing the oxidation of Xylitol to D-xylulose. Two copies of the XYL2 gene encoding XDH in the diploid yeast Candida tropicalis were sequentially disrupted using the Ura-blasting method. The XYL2-disrupted mutant, BSXDH-3, did not grow on a minimal medium containing D-Xylose as a sole carbon source. An enzyme assay experiment indicated that BSXDH-3 lost apparently all XDH activity. Xylitol production by BSXDH-3 was evaluated using a xylitol fermentation medium with glucose as a cosubstrate. As glucose was found to be an insufficient cosubstrate, various carbon sources were screened for efficient cofactor regeneration, and glycerol was found to be the best cosubstrate. BSXDH-3 produced xylitol with a volumetric productivity of 3.23 g liter(-1) h(-1) a specific productivity of 0.76 g g(-1) h(-1), and a xylitol yield of 98%. This is the first report of gene disruption of C. tropicalis for enhancing the efficiency of xylitol production. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.subject | RECOMBINANT SACCHAROMYCES-CEREVISIAE | - |
dc.subject | PICHIA-STIPITIS | - |
dc.subject | REDUCTASE | - |
dc.subject | CLONING | - |
dc.subject | TRANSFORMATION | - |
dc.subject | EXPRESSION | - |
dc.subject | ACIDS | - |
dc.title | Production of xylitol from D-xylose by a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis | - |
dc.type | Article | - |
dc.identifier.wosid | 000238620100050 | - |
dc.identifier.scopusid | 2-s2.0-33745170689 | - |
dc.type.rims | ART | - |
dc.citation.volume | 72 | - |
dc.citation.beginningpage | 4207 | - |
dc.citation.endingpage | 4213 | - |
dc.citation.publicationname | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.identifier.doi | 10.1128/AEM.02699-05 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Jung Hoe | - |
dc.contributor.nonIdAuthor | Byoung Sam Ko | - |
dc.contributor.nonIdAuthor | Jinmi Kim | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | RECOMBINANT SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | PICHIA-STIPITIS | - |
dc.subject.keywordPlus | REDUCTASE | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ACIDS | - |
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